Hui Wen-Tao, Ma Xiao-Bin, Zan Ying, Wang Xi-Jing, Dong Lei
Department of Gastroenterology, The Second Affiliated Hospital, Medical School of Xi'an Jiaotong University, Xi'an, Shaanxi 710000, China.
Chin Med J (Engl). 2015 Oct 5;128(19):2632-7. doi: 10.4103/0366-6999.166019.
MiR-34a dysregulation has been implicated in tumorigenesis and progression of gastric cancer, but its role in prognosis of patients with gastric cancer remains unknown. The aim of this study was to investigate the expression and prognostic significance of miR-34a in gastric cancer patients after radical gastrectomy.
Quantitative real-time polymerase chain reaction was performed to detect the expression of miR-34a in human gastric cancer cell lines and tissues in 76 patients with gastric adenocarcinoma from China. Results are assessed for association with clinical features and overall survival (OS) using Kaplan-Meier analysis. Prognostic values of miR-34a expression and clinical outcomes were evaluated by Cox regression analysis. A molecular prognostic stratification scheme incorporating miR-34a expression was determined using receiver operating characteristic analysis.
The results show that the expression level of miR-34a was decreased in human gastric cancer cell lines and tissues, and down-regulated expression of miR-34a was associated with Lauren classification (P = 0.034). Decreased miR-34a expression in gastric cancer tissues was positively correlated with poor OS of gastric cancer patients (P = 0.013). Further multivariate Cox regression analysis suggested that miR-34a expression was an independent prognostic indicator for gastric cancer (P = 0.027). Applying the prognostic value of miR-34a expression to tumor node metastasis (TNM) stage system showed a better prognostic value in patients with gastric cancer than miR-34a expression (P = 0.0435) or TNM stage (P = 0.0249) alone.
The results reinforce the critical role for the down-regulated miR-34a expression in gastric cancer and suggest that miR-34a could be a prognostic indicator for this disease.
miR-34a失调与胃癌的发生和进展有关，但其在胃癌患者预后中的作用尚不清楚。本研究旨在探讨miR-34a在胃癌根治术后患者中的表达及其预后意义。
采用定量实时聚合酶链反应检测76例中国胃腺癌患者的人胃癌细胞系和组织中miR-34a的表达。使用Kaplan-Meier分析评估结果与临床特征和总生存期（OS）的相关性。通过Cox回归分析评估miR-34a表达的预后价值和临床结局。使用受试者工作特征分析确定包含miR-34a表达的分子预后分层方案。
结果显示，miR-34a在人胃癌细胞系和组织中的表达水平降低，miR-34a表达下调与Lauren分类相关（P = 0.034）。胃癌组织中miR-34a表达降低与胃癌患者的不良OS呈正相关（P = 0.013）。进一步的多变量Cox回归分析表明，miR-34a表达是胃癌的独立预后指标（P = 0.027）。将miR-34a表达的预后价值应用于肿瘤淋巴结转移（TNM）分期系统，显示其对胃癌患者的预后价值优于单独的miR-34a表达（P = 0.0435）或TNM分期（P = 0.0249）。
结果强化了miR-34a表达下调在胃癌中的关键作用，并表明miR-34a可能是该疾病的预后指标。
