Gu Qiaoyan, Zhang Jun, Hu Haifeng, Tan Yu-E, Shi Shengmei, Nian Yuanyuan
Department of Gastroenterology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.
Department of Gastroenterology, The Affiliated Hospital of Yan'an University, Yan'an, China.
PLoS One. 2015 Nov 6;10(11):e0142377. doi: 10.1371/journal.pone.0142377. eCollection 2015.
The dysregulation of miR-137 plays vital roles in the oncogenesis and progression of various types of cancer, but its role in prognosis of gastric cancer patients remains unknown. This study was designed to investigate the expression and prognostic significance of miR-137 in gastric cancer patients after radical gastrectomy. Quantitative real-time PCR (qRT-PCR) was performed to evaluate the expression of miR-137 in human gastric cancer cell lines and tissues in patients with gastric adenocarcinoma. Results were assessed for association with clinical factors and overall survival by using Kaplan-Meier analysis. Prognostic values of miR-137 expression and clinical outcomes were evaluated by Cox regression analysis. The results exhibited that the expression level of miR-137 was decreased in human gastric cancer cell lines and tissues, and down-regulated expression of miR-137 was associated with tumor cell differentiation, N stage, and TNM stage. Decreased miR-137 expression in gastric cancer tissues was positively correlated with poor overall survival of gastric cancer patients. Further multivariate Cox regression analysis suggested that miR-137 expression was an independent prognostic indicator for gastric cancer except for TNM stage. Applying the prognostic value of miR-137 expression to TNM stage III group showed a better risk stratification for overall survival. In conclusion, the results reinforced the critical role for the down-regulated miR-137 expression in gastric cancer and suggested that miR-137 expression could be a prognostic indicator for this disease. In addition, these patients with TNM stage III gastric cancer and low miR-137 expression might need more aggressive postoperative treatment and closer follow-up.
miR-137的失调在各种类型癌症的发生和发展中起着至关重要的作用，但其在胃癌患者预后中的作用仍不清楚。本研究旨在探讨根治性胃切除术后胃癌患者中miR-137的表达及其预后意义。采用定量实时PCR（qRT-PCR）评估miR-137在胃腺癌细胞系和组织中的表达。通过Kaplan-Meier分析评估结果与临床因素及总生存期的相关性。通过Cox回归分析评估miR-137表达的预后价值和临床结局。结果显示，miR-137在人胃癌细胞系和组织中的表达水平降低，miR-137表达下调与肿瘤细胞分化、N分期及TNM分期相关。胃癌组织中miR-137表达降低与胃癌患者总生存期差呈正相关。进一步的多因素Cox回归分析表明，除TNM分期外，miR-137表达是胃癌的独立预后指标。将miR-137表达的预后价值应用于TNM III期组，显示出更好的总生存期风险分层。总之，结果强化了miR-137表达下调在胃癌中的关键作用，并表明miR-137表达可能是该疾病的预后指标。此外，这些TNM III期胃癌且miR-137表达低的患者可能需要更积极的术后治疗和更密切的随访。
