Trabert Britton, Xu Xia, Falk Roni T, Guillemette Chantal, Stanczyk Frank Z, McGlynn Katherine A
Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Department of Health and Human Services, Bethesda, MD 20892, USA.
Cancer Research Technology Program, Leidos Biomedical Research, Inc., Frederick National Laboratory for Cancer Research, Frederick, MD 21701, USA.
J Steroid Biochem Mol Biol. 2016 Jan;155(Pt A):56-62. doi: 10.1016/j.jsbmb.2015.09.032. Epub 2015 Sep 28.
Valid and precise measures of androgen concentrations are needed for etiologic studies of hormonally-related cancers. We developed a high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) method with two sample preparations to measure 11 androgens, including adrenal and gonadal androgenic precursors and their 5α-reduced metabolites.
Androgen levels were measured in serum from 20 healthy volunteers (5 men, 10 premenopausal women, 5 postmenopausal women). Two blinded, randomized aliquots per individual were assayed in each of three batches. A fourth batch of samples was measured at an external laboratory using comparable methodology to measure 9 of the 11 androgens. Coefficients of variation (CV) and intraclass correlation coefficients (ICC) were calculated from the individual components of variance. Comparability of 9 androgens across laboratories was assessed using Spearman ranked correlations, Deming regression and bias plots.
The laboratory CVs were <5% and ICCs were uniformly high (>95%) for all androgens measured across sex/menopausal status groups. Spearman ranked correlations for 9 hormones measured in the comparison laboratory were high (>0.85), suggesting good agreement.
Our high-performance LC-MS/MS assays of 11 androgens, including adrenal and gonadal androgenic precursors and their 5α-reduced metabolites demonstrated excellent laboratory reproducibility, and good comparability with an established method that measured 9 of the 11 hormones tested. The serum androgen metabolite assays are suitable for use in epidemiologic research.
对于激素相关癌症的病因学研究，需要有效且精确的雄激素浓度测量方法。我们开发了一种高效液相色谱-串联质谱（LC-MS/MS）方法，采用两种样品制备方法来测量11种雄激素，包括肾上腺和性腺雄激素前体及其5α-还原代谢产物。
对20名健康志愿者（5名男性、10名绝经前女性、5名绝经后女性）的血清中的雄激素水平进行测量。每个人的两份盲法随机等分试样在三个批次中分别进行检测。第四批样品在外部实验室使用可比方法进行检测，以测量11种雄激素中的9种。变异系数（CV）和组内相关系数（ICC）根据方差的各个组成部分进行计算。使用Spearman秩相关、Deming回归和偏差图评估不同实验室中9种雄激素的可比性。
在所有性别/绝经状态组中测量的所有雄激素，实验室CV均<5%，ICC均普遍较高（>95%）。在比较实验室中测量的9种激素的Spearman秩相关较高（>0.85），表明一致性良好。
我们对11种雄激素（包括肾上腺和性腺雄激素前体及其5α-还原代谢产物）的高效LC-MS/MS检测方法显示出出色的实验室可重复性，并且与一种测量所检测的11种激素中的9种的既定方法具有良好的可比性。血清雄激素代谢产物检测方法适用于流行病学研究。
