Hayashi Y, Nakagaki K, Nogami S, Hammerberg B, Tanaka H
College of Veterinary Medicine, University of Tokyo, Japan.
Am J Trop Med Hyg. 1989 Dec;41(6):650-6. doi: 10.4269/ajtmh.1989.41.650.
Protective immunity against infective larvae of Brugia malayi was studied in different strains of mice using various sources of antigens. The following strains of mice were susceptible to infective larvae development for 2 weeks after primary ip challenge: BALB/c, C3H/HeJ, C3H/NeN, C3H/HeJms, C57BL/6Jms, and DDD. In comparison to gerbils, BALB/c mice developed stronger resistance to infective larvae after immunization with irradiation attenuated larvae or with killed microfilariae (mf). However, killed mf failed to enhance resistance in C3H/HeJ mice, although C3H/HeN mice were strongly protected and C3H/HeJms mice were protected to a lesser degree by this antigen. Extracts of mf with phosphate buffered saline and sodium dodecyl sulfate both induced high levels of resistance in BALB/c mice. Transfer of resistance from BALB/c mice immunized with attenuated infective larvae to naive mice was accomplished at a high level at protection with nylon wool nonadherent spleen cells (T cells) but not with adherent cells treated with anti-Thy 1.2 serum and complement. In contrast, sera from immunized mice were much less protective.
利用各种抗原来源，在不同品系的小鼠中研究了针对马来布鲁线虫感染性幼虫的保护性免疫。以下品系的小鼠在初次腹腔注射攻击后2周内对感染性幼虫的发育敏感：BALB/c、C3H/HeJ、C3H/NeN、C3H/HeJms、C57BL/6Jms和DDD。与沙鼠相比，BALB/c小鼠在用辐射减毒幼虫或杀死的微丝蚴免疫后，对感染性幼虫产生了更强的抵抗力。然而，杀死的微丝蚴未能增强C3H/HeJ小鼠的抵抗力，尽管C3H/HeN小鼠受到了强烈保护，C3H/HeJms小鼠也受到了该抗原的一定程度保护。用磷酸盐缓冲盐水和十二烷基硫酸钠提取的微丝蚴提取物均在BALB/c小鼠中诱导了高水平的抵抗力。用尼龙棉非黏附脾细胞（T细胞）而不是用抗Thy 1.2血清和补体处理的黏附细胞，可将用减毒感染性幼虫免疫的BALB/c小鼠的抵抗力高水平转移至未免疫的小鼠。相比之下，免疫小鼠的血清保护作用要小得多。
