Tanaka M
Jpn J Exp Med. 1986 Aug;56(4):169-75.
A stable and long-lasting microfilaremia was developed by intravenous injection with 2 X 10(5) microfilariae (mf) of Brugia malayi into BALB/c mice. Mf density in mice was maintained at least at 30 to 40 mf/20 microliter blood for 4 weeks, then gradually decreased as the same as in the jird. However, mf inoculated were cleared from the blood within 3 weeks in the mice which was immunized with repeated intravenous injections with live mf. The effect of mf clearance was passively transferred into the mice with inoculated microfilaremia by intravenous injection of 0.2 ml serum from the immunized mice. For the analysis of the role of anti-mf antibodies, monoclonal antibodies (MABs) against mf of B. malayi were produced. An IgG1 MAB gave a significant mf reduction in mice and it promoted in vitro adherence of normal mouse spleen cells to mf.
通过向BALB/c小鼠静脉注射2×10⁵ 马来布鲁线虫微丝蚴（mf），建立了稳定且持久的微丝蚴血症。小鼠体内的mf密度至少在30至40 mf/20微升血液中维持4周，然后像沙鼠一样逐渐下降。然而，用活mf反复静脉注射免疫的小鼠，接种的mf在3周内从血液中清除。通过静脉注射0.2 ml来自免疫小鼠的血清，mf清除效果被被动转移到接种有微丝蚴血症的小鼠体内。为了分析抗mf抗体的作用，制备了抗马来布鲁线虫mf的单克隆抗体（MABs）。一种IgG1 MAB在小鼠体内使mf显著减少，并促进正常小鼠脾细胞在体外与mf的黏附。
