Hitch W L, Lammie P J, Eberhard M L
Tulane University, New Orleans, Louisiana.
Am J Trop Med Hyg. 1989 Dec;41(6):657-63. doi: 10.4269/ajtmh.1989.41.657.
The immunological consequences of exposure to filarial infection were examined by cross-sectional serological studies. Serum samples from 121 pediatric patients (18 months-15 years of age) were analyzed in parallel with a panel of sera from adults residing in the same area of Haiti. Parasite antigen specific IgG and IgE levels were determined by ELISA. IgG levels in children were significantly elevated in humoral immunoreactivity to Brugia pahangi extracts compared to adults. In addition, anti-filarial IgG levels in amicrofilaremic children were significantly greater than in microfilaremic children. In contrast, IgG levels in adults were equivalent independent of microfilaremic status. Anti-filarial IgE levels in sera from both children and adults were low in comparison to that of a subject with tropical pulmonary eosinophilia and were unrelated to clinical status. No correlations were found between humoral responses and age, sex, or degree of parasitemia. Sera from amicrofilaremic children and, to a lesser extent, adults recognize more antigens, particularly those of high molecular weight (greater than 55 kDa), than sera from microfilaremic patients.
通过横断面血清学研究来检测接触丝虫感染的免疫学后果。对121名儿科患者（18个月至15岁）的血清样本与居住在海地同一地区的一组成人血清进行平行分析。通过酶联免疫吸附测定法（ELISA）测定寄生虫抗原特异性IgG和IgE水平。与成人相比，儿童对彭亨布鲁线虫提取物的体液免疫反应中IgG水平显著升高。此外，无微丝蚴血症儿童的抗丝虫IgG水平显著高于有微丝蚴血症的儿童。相比之下，成人的IgG水平与微丝蚴血症状态无关。与热带肺嗜酸性粒细胞增多症患者相比，儿童和成人血清中的抗丝虫IgE水平较低，且与临床状态无关。未发现体液反应与年龄、性别或寄生虫血症程度之间存在相关性。无微丝蚴血症儿童的血清，以及在较小程度上成人的血清，比有微丝蚴血症患者的血清能识别更多抗原，特别是那些高分子量（大于55 kDa）的抗原。
