Rodríguez-Sánchez Irám Pablo, Treviño-Alvarado Víctor Manuel, Torres-Sepúlveda María del Rosario, López-Saldaña Liliana Aracely, Ponce-García Gustavo, López-Uriarte Graciela Areli, Ruiz-Herrera María del Consuelo, Zamora-Ávila Diana Elisa, Villarreal-Pérez Jesús Zacarías, Dávalos-Aranda Guillermo, Martínez-de-Villarreal Laura Elia
Departamento de Genética, Hospital Universitario "Dr. José Eleuterio González", Universidad Autónoma de Nuevo León, Monterrey, Nuevo León, Mexico.
Tecnológico de Monterrey Cátedra de Bioinformática, Campus Monterrey, Monterrey, Nuevo León, Mexico.
Acta Vet Scand. 2015 Sep 29;57:62. doi: 10.1186/s13028-015-0144-9.
Free amino acids and acylcarnitines circulating in the blood can be used for diagnosis for metabolic illness and imbalances. To date, the normal reference ranges of amino acids and acylcarnitines in horse peripheral blood have not been established. In this study, the concentrations of 12 amino acids and 26 acylcarnitines were determined by tandem mass spectrometry in complete blood from 100 healthy horses (50 Quarter horses (QH) [23 males and 27 females] and 50 American Miniature horses (AMH) [15 males and 35 females]) with no signs of metabolic disease. The means and standard deviations were determined and data statistically analyzed.
Concentrations of short, medium, and long chain acylcarnitines were significantly higher in male AMH than in male QH. The concentrations of the amino acids alanine, arginine, glycine, proline (glycogenic), and leucine (ketogenic) were higher in the QH than in the AMH. Female AMH had higher concentrations of propionylcarnitine, leucine, proline, arginine, and ornithine than female QH.
Normal reference ranges of amino acids and acylcarnitines were established for AMH and QH. Significant differences were found in concentration of these compounds between breeds and gender.
血液中循环的游离氨基酸和酰基肉碱可用于代谢疾病和失衡的诊断。迄今为止，马外周血中氨基酸和酰基肉碱的正常参考范围尚未确定。在本研究中，通过串联质谱法测定了100匹无代谢疾病迹象的健康马（50匹夸特马[23匹雄性和27匹雌性]和50匹美国迷你马[15匹雄性和35匹雌性]）全血中12种氨基酸和26种酰基肉碱的浓度。确定了平均值和标准差，并对数据进行了统计分析。
雄性美国迷你马中短链、中链和长链酰基肉碱的浓度显著高于雄性夸特马。夸特马中丙氨酸、精氨酸、甘氨酸、脯氨酸（生糖氨基酸）和亮氨酸（生酮氨基酸）的浓度高于美国迷你马。雌性美国迷你马中丙酰肉碱、亮氨酸、脯氨酸、精氨酸和鸟氨酸的浓度高于雌性夸特马。
确定了美国迷你马和夸特马氨基酸和酰基肉碱的正常参考范围。发现这些化合物的浓度在品种和性别之间存在显著差异。
