Fu Caili, Yang Dongying, Peh Wan Yi Elaine, Lai Shaojuan, Feng Xiao, Yang Hongshun
Food Science and Technology Programme, Dept. of Chemistry, National Univ. of Singapore, 3 Science Drive 3, Singapore, 117543, Republic of Singapore.
College of Bioscience & Biotechnology, Fuzhou Univ, Fuzhou, 350108, China.
J Food Sci. 2015 Oct;80(10):C2191-9. doi: 10.1111/1750-3841.13005. Epub 2015 Sep 28.
Proanthocyanidins were isolated and purified from fruits of elephant apple (Dillenia indica Linn.) and their structural and bioactive properties were examined. Bate-Smith alcoholysis, FTIR, and (13) C NMR spectra revealed that elephant apple proanthocyanidins (EAPs) contained a dominant amount of B-type procyanidins (PC) with a minor amount of B-type prodelphinidins (PD) but no A-type interflavan linkage. (13) C NMR spectrum indicated that the cis isomer was dominant in EAPs. The electron spray ionization and matrix-assisted laser desorption ionization time of flight mass spectra of EAPs showed the clear ion peaks corresponding to B-type PC dimer to B-type PD with degree of polymerization of 11. EAPs had strong antioxidant activity, which was evidenced by the high oxygen radical scavenging capacity at 1.06 × 10(4) μmol TE/g and ferric reducing antioxidant power of 2320 μmol Fe(II)/g. The results suggest that EAPs could be extracted to be used as promising functional food materials.
In this study, the elephant apple proanthocyanidins (EAPs) with a yield of 0.23% were identified for the first time as dominant B-type poly(catechin/epicatechin) but no A-type interflavan linkage. EAPs had higher ORAC and FRAP values compared to commercial grape seed proanthocyanidins, suggesting that EAPs may be used as promising functional food materials.
从象苹果（锡兰天料木）果实中分离纯化原花青素，并对其结构和生物活性进行研究。贝特 - 史密斯醇解、傅里叶变换红外光谱和碳 - 13核磁共振光谱表明，象苹果原花青素（EAPs）主要含有B型原花青素（PC），少量B型原翠雀素（PD），但不存在A - 型黄烷间键。碳 - 13核磁共振光谱表明顺式异构体在EAPs中占主导地位。EAPs的电喷雾电离和基质辅助激光解吸电离飞行时间质谱显示出清晰的离子峰，对应于聚合度为11的B型PC二聚体到B型PD。EAPs具有很强的抗氧化活性，高氧自由基清除能力为1.06×10⁴ μmol TE/g，铁还原抗氧化能力为2320 μmol Fe(II)/g，证明了这一点。结果表明，EAPs可提取用作有前景的功能性食品原料。
在本研究中，首次鉴定出象苹果原花青素（EAPs），其产量为0.23%，主要为B型聚（儿茶素/表儿茶素），但不存在A - 型黄烷间键。与市售葡萄籽原花青素相比，EAPs具有更高的氧自由基吸收能力（ORAC）和铁还原抗氧化能力（FRAP）值，表明EAPs可用作有前景的功能性食品原料。
