Chen Xiao-Xin, Liang Ge, Chai Wei-Ming, Feng Hui-Ling, Zhou Han-Tao, Shi Yan, Chen Qing-Xi
School of Life Sciences, Xiamen University, Xiamen 361005, Fujian, China.
School of Life Sciences, Xiamen University, Xiamen 361005, Fujian, China.
J Biosci Bioeng. 2014 Nov;118(5):583-7. doi: 10.1016/j.jbiosc.2014.04.015. Epub 2014 May 24.
In the present study the structure of proanthocyanidins from Polyalthia longifolia leaves was characterized with (13)C nuclear magnetic resonance, high performance liquid chromatography electrospray ionization mass spectrometry, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analyses. The results showed that the proanthocyanidins were mixture of homopolymers of B-type procyanidins with degree of polymerization up to 14-mer. Furthermore, the antioxidant activity of the proanthocyanidins was studied through 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) free-radical scavenging activities, and ferric reducing/antioxidant power assays. In addition, antityrosinase activity of the proanthocyanidins was investigated. The IC50 for 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) free-radical scavenging activity of the proanthocyanidins were 89.32 ± 12.07 and 76.79 ± 5.88 μg/mL, respectively; the ferric reducing/antioxidant power value was 710.54 ± 142.82 mg ascorbic acid equivalent/g dry weight. The IC50 for antityrosinase activity was 773.09 ± 1.47 μg/mL. In conclusion, the proanthocyanidins from P. longifolia leaves exhibited potent antioxidant and antityrosinase activities. This research would provide scientific evidence for the use of proanthocyanidins from P. longifolia leaves as antioxidant and antityrosinase agents.
在本研究中，通过碳-13核磁共振、高效液相色谱-电喷雾电离质谱以及基质辅助激光解吸/电离飞行时间质谱分析对长叶暗罗叶中原花青素的结构进行了表征。结果表明，原花青素是聚合度高达14聚体的B型原花青素同聚物的混合物。此外，通过2,2-二苯基-1-苦基肼和2,2'-联氮-双-(3-乙基苯并噻唑啉-6-磺酸)自由基清除活性以及铁还原/抗氧化能力测定研究了原花青素的抗氧化活性。另外，还研究了原花青素的抗酪氨酸酶活性。原花青素对2,2-二苯基-1-苦基肼和2,2'-联氮-双-(3-乙基苯并噻唑啉-6-磺酸)自由基清除活性的半数抑制浓度分别为89.32±12.07和76.79±5.88μg/mL；铁还原/抗氧化能力值为710.54±142.82mg抗坏血酸当量/克干重。抗酪氨酸酶活性的半数抑制浓度为773.09±1.47μg/mL。总之，长叶暗罗叶中的原花青素表现出较强的抗氧化和抗酪氨酸酶活性。本研究将为长叶暗罗叶中的原花青素用作抗氧化剂和抗酪氨酸酶剂提供科学依据。
