Karakoca Kubra, Ozusaglam Meltem Asan, Cakmak Yavuz Selim, Erkul Seher Karaman
Department of Biology, Faculty of Arts and Sciences, Aksaray University, Aksaray, Turkey.
Department of Biotechnology and Molecular Biology, Faculty of Arts and Sciences, Aksaray University, Aksaray, Turkey.
EXCLI J. 2013 Feb 28;12:150-67. eCollection 2013.
The Isatis species has antibacterial, anticancer and antiviral properties and these important endemic plants grow widely in various parts of Anatolian-Turkey. In the present study, the antioxidant activities of Isatis floribunda Boiss. ex Bornm. flower and root extracts were determined with total antioxidant activity, free radical scavenging activity, ferric ion reducing power, and cupric ion reducing antioxidant capacity assay. The total phenolic compounds and flavonoids were also examined for the extracts. The antimicrobial activities of the extracts were investigated by using the disc diffusion and microdilution-broth methods against human and fish pathogen microorganisms. The phenolic composition of I. floribunda root methanolic extract was analyzed by high performance liquid chromatography (HPLC). The major component in the extract was cholorogenic acid (1980.20 µg/g). The cytotoxic effect of the methanolic root extract was also tested on human breast cell (MCF12A). The results showed that I. floribunda could be used as a natural source in the food and feed industry and clinical and food chemistry, and that the antimicrobial agents could be used against human and fish pathogens.
菘蓝属植物具有抗菌、抗癌和抗病毒特性，这些重要的本土植物广泛生长在土耳其安纳托利亚的各个地区。在本研究中，采用总抗氧化活性、自由基清除活性、铁离子还原能力和铜离子还原抗氧化能力测定法，对多花菘蓝（Isatis floribunda Boiss. ex Bornm.）花和根提取物的抗氧化活性进行了测定。还对提取物中的总酚类化合物和黄酮类化合物进行了检测。采用纸片扩散法和微量稀释肉汤法，研究了提取物对人和鱼类病原微生物的抗菌活性。采用高效液相色谱法（HPLC）分析了多花菘蓝根甲醇提取物的酚类成分。提取物中的主要成分是绿原酸（1980.20 µg/g）。还测试了根甲醇提取物对人乳腺细胞（MCF12A）的细胞毒性作用。结果表明，多花菘蓝可作为食品和饲料工业以及临床和食品化学中的天然来源，其抗菌剂可用于对抗人和鱼类病原体。
