Ozusaglam Meltem Asan, Darilmaz Derya Onal, Erzengin Mahmut, Teksen Mehtap, Erkul Seher Karaman
Department of Biotechnology and Molecular Biology, Faculty of Art and Science, Aksaray University, Aksaray, Turkey.
Afr J Tradit Complement Altern Med. 2013 Apr 12;10(3):449-57. doi: 10.4314/ajtcam.v10i3.11. eCollection 2013.
This study was designed to examine the in vitro antimicrobial and antioxidant activities of the methanol, ethanol, water, n-hexane and dicholoromethane extracts of two Allium species (Allium scabriflorum and Allium tchihatschewii) which are endemic for the flora of Turkey. The antimicrobial efficiency of the plant was evaluated according to disc diffusion and microdilution broth methods. The antimicrobial test results showed that the extracts of A. scabriflorum and A. tchihatschewii showed varying degrees of antimicrobial activity on the tested microorganisms. The extracts were screened for their possible antioxidant activities by three complementary tests; DPPH free radical-scavenging, scavenging of hydrogen peroxide and metal chelating activity assays. All the extracts of A. scabriflorum and A. tchihatschewii exhibited lower DPPH free radical scavenging activity but higher metal chelating activity when compared to standards. The values of scavenging of hydrogen peroxide of the extracts were higher than the standards that of α-tocopherol, BHA, BHT and trolox, but close to that of ascorbic acid. In addition to the antioxidant activity of these plants, the total phenolic compounds and flavonoids were also measured in the extracts. The results presented here may suggest that the extracts of A. scabriflorum and A. tchihatschewii possess antimicrobial and antioxidant properties, and therefore, they can be used as a natural preservative ingredient in food and/or pharmaceutical industry.
本研究旨在检测两种葱属植物(糙花葱和茨哈切夫葱)的甲醇、乙醇、水、正己烷和二氯甲烷提取物的体外抗菌和抗氧化活性,这两种植物是土耳其植物区系中的特有物种。根据纸片扩散法和微量稀释肉汤法评估植物的抗菌效率。抗菌测试结果表明,糙花葱和茨哈切夫葱的提取物对受试微生物表现出不同程度的抗菌活性。通过三种互补试验筛选提取物的潜在抗氧化活性;DPPH自由基清除、过氧化氢清除和金属螯合活性测定。与标准品相比,糙花葱和茨哈切夫葱的所有提取物均表现出较低的DPPH自由基清除活性,但具有较高的金属螯合活性。提取物的过氧化氢清除值高于α-生育酚、丁基羟基茴香醚、二丁基羟基甲苯和特洛克斯的标准值,但接近抗坏血酸的标准值。除了这些植物的抗氧化活性外,还测定了提取物中的总酚类化合物和黄酮类化合物。此处给出的结果可能表明,糙花葱和茨哈切夫葱的提取物具有抗菌和抗氧化特性,因此,它们可作为食品和/或制药行业的天然防腐剂成分。