Sailaja K V, Shivaranjani V Leela, Poornima H, Rahamathulla S B Md, Devi K Lakshmi
Department of Biochemistry, Sri Krishnadevaraya University, Anantapur-515003,Andhra Pradesh, India.
EXCLI J. 2013 May 6;12:373-83. eCollection 2013.
The objective of the present study was to evaluate the possible protective effects of Tribulus terrestris fruit aqueous extract (TTFAEt) on lipid profile and oxidative stress in isoproterenol (ISO) induced myocardial necrosis in albino Wistar rats. Albino Wistar rats were divided into normal control, TTFAEt alone treated, ISO control and pretreated (TTFAEt+ISO) groups. The extract was administered at a dose of 50 mg/kg body weight for 40 days orally by gavage and ISO was administered at a dose of 85 mg/kg body weight for two consecutive days intraperitoneally at an interval of 24 h. ISO induced myocardial infarction (MI) was confirmed by disturbances in serum lipid profile, heart tissue lipid peroxidation and antioxidant enzyme levels. There was a significant increase in the levels of serum total cholesterol (32.60 %), triglycerides (41.30 %), very low density lipoproteins (81.81 %), low density lipoproteins (84%) and phospholipids (38.88 %) and a significant decrease in the levels of high density lipoproteins (33.33 %) in the ISO control group when compared to normal controls. Additionally, there is a significant decrease in the levels of heart tissue antioxidant enzymes such as superoxide dismutase, catalase, glutathione reductase, glutathione peroxidase and depletion of reduced glutathione, which indicates enhanced lipid peroxidation(172 %). Pretreatment with extract significantly showed a protective effect against ISO altered lipid profile, lipid peroxidation and antioxidant enzyme levels. The present study showed therapeutic effect of TTFAEt on lipid profile and oxidative stress in isoproterenol (ISO) induced myocardial necrosis in experimental rats.
本研究的目的是评估刺蒺藜果实水提取物（TTFAEt）对异丙肾上腺素（ISO）诱导的白化Wistar大鼠心肌坏死中脂质谱和氧化应激的可能保护作用。将白化Wistar大鼠分为正常对照组、单独使用TTFAEt治疗组、ISO对照组和预处理（TTFAEt + ISO）组。提取物以50 mg/kg体重的剂量通过灌胃口服给药40天，ISO以85 mg/kg体重的剂量连续两天腹腔注射，间隔24小时。通过血清脂质谱、心脏组织脂质过氧化和抗氧化酶水平的紊乱证实了ISO诱导的心肌梗死（MI）。与正常对照组相比，ISO对照组血清总胆固醇（32.60%）、甘油三酯（41.30%）、极低密度脂蛋白（81.81%）、低密度脂蛋白（84%）和磷脂（38.88%）水平显著升高，高密度脂蛋白（33.33%）水平显著降低。此外，心脏组织抗氧化酶如超氧化物歧化酶、过氧化氢酶、谷胱甘肽还原酶、谷胱甘肽过氧化物酶水平显著降低，还原型谷胱甘肽耗竭，这表明脂质过氧化增强（172%）。提取物预处理显著显示出对ISO改变的脂质谱、脂质过氧化和抗氧化酶水平的保护作用。本研究表明TTFAEt对实验大鼠异丙肾上腺素（ISO）诱导的心肌坏死中的脂质谱和氧化应激具有治疗作用。
