Adedeji Adebayo Lawrence, Adenikinju Rufus Omotayo, Ajele Joshua Olufemi, Olawoye Theophilus Ladapo
Department of Biochemistry, Ladoke Akintola University of Technology, Ogbomoso, Nigeria ; Department of Biochemistry, Federal University of Technology, Akure, Nigeria University.
Health Centre, Federal University of Technology, Akure, Nigeria.
EXCLI J. 2014 Jul 15;13:761-71. eCollection 2014.
In this report, we compared the serum protein electrophoresis (SPE) patterns in a subset of HIV-1-infected subjects who did not progress to AIDS without antiretroviral treatment with those in whose control of disease progression was achieved by highly active antiretroviral therapy (HAART). SPE and immunofixation electrophoresis were performed on Helena Electrophoresis System according to manufacturer's instructions. The percentage of SPE abnormalities, resembling chronic inflammation, was significantly higher in HIV-1-infected subject without HAART compared with those under HAART (p = 0.001). The majority of individuals under HAART showed evidence of oligoclonal bands on the γ-band against a polyclonal background compared with those without HAART but ß-γ-band bridging was more evident. Immunofixation pattern was consistent with oligoclonal hypergammaglobulinaemia of IgG kappa type, which was found to be more intense in group without HAART. HIV clinical status did not show appreciable effect on the SPE pattern in subjects without HAART. However, under effective HAART, subjects with better CD4 T-cell count were associated with higher γ-globulin band. In group without HAART, acute infection was found to be associated the higher γ-globulin fraction compared with chronic infection. The opposite was the case under effective HAART. HIV infected subjects that did not progress to AIDS were associated with markedly abnormal SPE pattern. Overall results reflect the host ability compensate defective cellular immunity in HIV-1 infection with humoral immune responses. These findings underscore the usefulness of SPE monitoring HIV disease management and identifying individuals that may not progress to full-blown AIDS in the absence of treatment.
在本报告中，我们比较了一组未经抗逆转录病毒治疗但未进展为艾滋病的HIV-1感染受试者与通过高效抗逆转录病毒疗法（HAART）实现疾病进展控制的受试者的血清蛋白电泳（SPE）图谱。根据制造商的说明，在海伦娜电泳系统上进行SPE和免疫固定电泳。与接受HAART治疗的受试者相比，未接受HAART治疗的HIV-1感染受试者中类似慢性炎症的SPE异常百分比显著更高（p = 0.001）。与未接受HAART治疗的受试者相比，大多数接受HAART治疗的个体在γ带出现寡克隆带的证据，但β-γ带桥接更明显。免疫固定图谱与IgG κ型寡克隆高丙种球蛋白血症一致，在未接受HAART治疗的组中发现更为强烈。HIV临床状态对未接受HAART治疗的受试者的SPE图谱没有明显影响。然而，在有效的HAART治疗下，CD4 T细胞计数较好的受试者与较高的γ球蛋白带相关。在未接受HAART治疗的组中，发现急性感染与较高的γ球蛋白分数相关，而慢性感染则相反。在有效的HAART治疗下情况则相反。未进展为艾滋病的HIV感染受试者与明显异常的SPE图谱相关。总体结果反映了宿主通过体液免疫反应补偿HIV-1感染中缺陷细胞免疫的能力。这些发现强调了SPE在监测HIV疾病管理和识别在无治疗情况下可能不会进展为全面艾滋病的个体方面的有用性。
