Srivastava Vikas, Verma Praveen Kumar
Plant Immunity Laboratory, National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi, India.
PLoS One. 2015 Sep 29;10(9):e0138719. doi: 10.1371/journal.pone.0138719. eCollection 2015.
The eukaryotic lineage-specific LIM protein (LIN11, ISL1, and MEC3) family play pivotal role in modulation of actin dynamics and transcriptional regulation. The systematic investigation of this family has not been carried in detail and rare in legumes. Current study involves the mining of Cicer arietinum genome for the genes coding for LIM domain proteins and displayed significant homology with LIM genes of other species. The analysis led to the identification of 15 members, which were positioned on chickpea chromosomes. The phylogenetic and motif analysis suggested their categorization into two sub-families i.e., Ca-2LIMs and Ca-DA1/DAR, which comprised of nine and six candidates, respectively. Further sub-categories of Ca-2LIMs were recognised as αLIM, βLIM, δLIM and γLIM. The LIM genes within their sub-families displayed conserved genomic and motif organization. The expression pattern of Ca-2LIMs across developmental and reproductive tissues demonstrated strong correlation with established consensus. The Ca-2LIM belongs to PLIM and GLIM (XLIM) was found highly expressed in floral tissue. Others showed ubiquitous expression pattern with their dominance in stem. Under hormonal and pathogenic conditions these LIMs were found to up-regulate during salicylic acid, abscisic acid and Ascochyta rabiei treatment or infection; and down-regulated in response to jasmonic acid treatment. The findings of this work, particularly in terms of modulation of LIM genes under biotic stress will open up the way to further explore and establish the role of chickpea LIMs in plant defense response.
真核生物谱系特异性LIM蛋白（LIN11、ISL1和MEC3）家族在肌动蛋白动力学调节和转录调控中起关键作用。对该家族的系统研究尚未详细开展，在豆科植物中也较为罕见。当前的研究涉及在鹰嘴豆基因组中挖掘编码LIM结构域蛋白的基因，这些基因与其他物种的LIM基因具有显著的同源性。分析鉴定出了15个成员，它们定位在鹰嘴豆染色体上。系统发育和基序分析表明，它们可分为两个亚家族，即Ca-2LIMs和Ca-DA1/DAR，分别包含9个和6个候选成员。Ca-2LIMs的进一步亚类被识别为αLIM、βLIM、δLIM和γLIM。其亚家族内的LIM基因显示出保守的基因组和基序组织。Ca-2LIMs在发育和生殖组织中的表达模式与已确立的共识高度相关。属于PLIM和GLIM（XLIM）的Ca-2LIM在花组织中高度表达。其他成员则表现出普遍的表达模式，在茎中占主导地位。在激素和致病条件下，这些LIMs在水杨酸、脱落酸和鹰嘴豆壳二孢菌处理或感染期间被上调；而在茉莉酸处理时则被下调。这项工作的发现，特别是在生物胁迫下LIM基因的调控方面，将为进一步探索和确定鹰嘴豆LIMs在植物防御反应中的作用开辟道路。
