Department of Plant Sciences, Central University of Jammu, Rahya-Suchani (Bagla), Dist. Samba, Jammu, Jammu and Kashmir, 181143, India.
Plant Immunity Laboratory, National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi, India.
Planta. 2017 Sep;246(3):365-375. doi: 10.1007/s00425-017-2715-7. Epub 2017 Jun 17.
The plant LIMs comprise two sub-families with one (DA1/DAR) and two (2LIM) LIM domains. This review comprehensively discussed the structure and potential role of this protein family in diverse area of plant biology. The description of first eukaryote lineage-specific plant LIM domain (LIN11, ISL1, and MEC3) proteins was observed in Helianthus long back. The successive study of LIM proteins in diverse plants has shown its vital relation to development, metabolism and defence. This nascent gene family has been worked out for their role in actin dynamics, organ size determination and transcription regulation. On grounds of protein architecture, two sub-families have been delineated as DA1/DAR (one LIM domain) and 2LIMs (two LIM domains). The genomic and expression study guides to the identification of diverse sub-categories. The significance of 2LIMs in regulation of actin dynamics leading to pollen growth and development has prospects to understand the plant reproductive behaviour. Interestingly, new facet of these LIMs as a transcriptional regulator in biological pathway/biosynthesis was also reported. Recently, the cumulative contribution of these features was also recognized for obtaining good quality fibre, thus giving translational outlook to this family. The DA1/DAR proteins are orchestrated with additional domains and provide a key role in regulation of organ size and tolerance to biotic and abiotic stress. This review will focus the journey of plant LIMs till date and will cover details of its structure, type, classification and functional relevance. This will provide insight to identify the potential of this gene family in the improvement of desired crop features.
植物 LIM 蛋白包含两个亚家族，分别具有一个（DA1/DAR）和两个（2LIM）LIM 结构域。本文全面讨论了该蛋白家族在植物生物学各个领域的结构和潜在作用。首次在向日葵中观察到第一个真核生物谱系特异性植物 LIM 结构域（LIN11、ISL1 和 MEC3）蛋白。随后在不同植物中对 LIM 蛋白的研究表明，它与发育、代谢和防御密切相关。这个新兴的基因家族因其在肌动蛋白动力学、器官大小决定和转录调控中的作用而得到研究。根据蛋白质结构，将两个亚家族划分为 DA1/DAR（一个 LIM 结构域）和 2LIMs（两个 LIM 结构域）。基因组和表达研究有助于鉴定不同的亚类。2LIMs 在调节肌动蛋白动力学以促进花粉生长和发育方面的重要性，有望深入了解植物生殖行为。有趣的是，这些 LIMs 作为生物途径/生物合成中的转录调节剂的新方面也有报道。最近，人们还认识到这些特征在获得优质纤维方面的综合贡献，从而为该家族提供了转化前景。DA1/DAR 蛋白与其他结构域协同作用，在调节器官大小和对生物和非生物胁迫的耐受性方面发挥关键作用。本文将重点介绍植物 LIMs 迄今为止的研究进展，涵盖其结构、类型、分类和功能相关性的详细信息。这将有助于确定该基因家族在改善所需作物特征方面的潜力。
