Widespread nuclease contamination in commonly used oxygen-scavenging systems.
作者信息
Senavirathne Gayan, Liu Jiaquan, Lopez Miguel A, Hanne Jeungphill, Martin-Lopez Juana, Lee Jong-Bong, Yoder Kristine E, Fishel Richard
机构信息
Department of Molecular Virology, Immunology and Medical Genetics, The Ohio State University Medical Center, Columbus, Ohio, USA.
Department of Physics, Pohang University of Science and Technology, Pohang, Korea.
出版信息
Nat Methods. 2015 Oct;12(10):901-2. doi: 10.1038/nmeth.3588.
Abstract
摘要
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Acidification of the oxygen scavenging system in single-molecule fluorescence studies: in situ sensing with a ratiometric dual-emission probe.在单分子荧光研究中氧气清除系统的酸化：用比率双发射探针进行原位检测。
Anal Chem. 2010 Jul 15;82(14):6132-8. doi: 10.1021/ac1008749.
3
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4
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5
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