Liu Zhangshun, Liu Jie, Shi Xiaohong, Wang Lihong, Yang Yan, Tao Minfang
Department of Reproductive Medicine Center, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, No. 600 Yishan Road, Shanghai, 200233, China.
Reprod Biol Endocrinol. 2015 Sep 30;13:111. doi: 10.1186/s12958-015-0107-z.
Level of the testosterone in a man's life span is very important. Studies on the serum testosterone concentrations in different age groups of healthy men were controversial. The aim of this study was to investigate dynamic changes of serum reproductive hormones with aging in healthy Chinese male and to compare its correlation with age.
Total of 1,093 healthy Chinese men from Shanghai aged from 20 to 87 years old was enrolled in the study. Concentrations of serum total testosterone (T), luteinizing hormone (LH) and sex hormone binding globulin (SHBG) were quantified by EIA. Testosterone secretion index (TSI) and free testosterone index (FTI) were then calculated. Data were analyzed by SPSS program. Non-parametric tests and univariate linear regression analyses were used.
The 1,039 male participants were grouped into 12 groups by 5-year apart for each group. Significant differences in T, LH, SHBG, FTI and TSI were found between the 12 different age groups. Average of serum total T was 15.36 ± 4.86 nmol/L; LH was 4.76 ± 2.76 IU/L, SHBG was 32.61 ± 17.24 nmol/L. Compared to age 20 ~ 24 group, serum T level of age 35 ~ 39, 40 ~ 44, 45 ~ 49, 50 ~ 54, and 55 ~ 59 was significantly decreased (p < 0.05). Intriguingly, however, serum T level of age 60 or older did not significantly reduced compared to the age of 20 ~ 24 group. Serum LH and SHBG were positively correlated with aging (p <0.01), while TSI and FTI were negatively correlated with aging (p <0.01). In addition, BMI was negatively and significantly correlated with levels of T (r = -0.585, p < 0.001), LH (r = -0.090, p < 0.001), SHBG (r = - 1.817, p < 0.001), and TSI (r = - 0.104, p < 0.001), but positively and significantly correlated with FTI level (r = 0.011, p < 0.001).
Serum total testosterone fluctuated with aging in adult men, and FTI and TSI decreased gradually with aging. While age was not significantly correlated with T level, BMI was significantly and negatively correlated with T level, suggesting body weight may affect testosterone level.
男性一生中睾酮水平非常重要。关于健康男性不同年龄组血清睾酮浓度的研究存在争议。本研究旨在探讨健康中国男性血清生殖激素随年龄增长的动态变化，并比较其与年龄的相关性。
本研究纳入了1093名来自上海的20至87岁健康中国男性。采用酶免疫分析法（EIA）测定血清总睾酮（T）、促黄体生成素（LH）和性激素结合球蛋白（SHBG）的浓度。然后计算睾酮分泌指数（TSI）和游离睾酮指数（FTI）。数据采用SPSS软件进行分析，使用非参数检验和单变量线性回归分析。
1039名男性参与者按每5岁一组分为12组。12个不同年龄组的T、LH、SHBG、FTI和TSI存在显著差异。血清总T的平均值为15.36±4.86nmol/L；LH为4.76±2.76IU/L，SHBG为32.61±17.24nmol/L。与20至24岁组相比，35至39岁、40至44岁、45至49岁、50至54岁和55至59岁组的血清T水平显著降低（p<0.05）。然而，有趣的是，60岁及以上组的血清T水平与20至24岁组相比并未显著降低。血清LH和SHBG与年龄呈正相关（p<0.01），而TSI和FTI与年龄呈负相关（p<0.01）。此外，体重指数（BMI）与T水平（r=-0.585，p<0.001）、LH水平（r=-0.090，p<0.001）、SHBG水平（r=-1.817，p<0.001）和TSI水平（r=-0.104，p<0.001）呈显著负相关，但与FTI水平呈显著正相关（r=0.011，p<0.001）。
成年男性血清总睾酮随年龄增长而波动，FTI和TSI随年龄增长逐渐降低。虽然年龄与T水平无显著相关性，但BMI与T水平呈显著负相关，提示体重可能影响睾酮水平。
