Moravcová Simona, Červená Kateřina, Pačesová Dominika, Bendová Zdeňka
Department of Physiology, Faculty of Science, Charles University in Prague, Prague, Czech Republic.
J Neurosci Res. 2016 Jan;94(1):99-108. doi: 10.1002/jnr.23673. Epub 2015 Sep 30.
Signal transducers and activators of transcription (STAT) proteins regulate many aspects of cellular physiology from growth and differentiations to immune responses. Using immunohistochemistry, we show the daily rhythm of STAT3 protein in the rat suprachiasmatic nucleus (SCN), with low but significant amplitude peaking in the morning. We also reveal the strong expression of STAT5A in astrocytes of the SCN and the STAT5B signal in nonastrocytic cells. Administration of lipopolysaccharide (LPS) acutely induced phosphorylation of STAT3 on Tyr705 during both the day and the night and induced phosphorylation on Ser727 but only after the daytime application. The LPS-induced phospho-STAT3 (Tyr705) remained elevated for 24 hr after the daytime application but declined within 8 hr when LPS was applied at night.
信号转导子和转录激活子（STAT）蛋白调节细胞生理学的许多方面，从生长、分化到免疫反应。利用免疫组织化学方法，我们展示了大鼠视交叉上核（SCN）中STAT3蛋白的每日节律，其振幅较低但在早晨达到显著峰值。我们还揭示了SCN星形胶质细胞中STAT5A的强烈表达以及非星形胶质细胞中的STAT5B信号。脂多糖（LPS）给药在白天和夜间均急性诱导STAT3在Tyr705位点的磷酸化，并诱导Ser727位点的磷酸化，但仅在白天给药后出现。白天应用LPS后，LPS诱导的磷酸化STAT3（Tyr705）在24小时内保持升高，但在夜间应用LPS时，其在8小时内下降。
