Shi Xin, Gao Rui, Ying Yi-Lun, Si Wei, Chen Yunfei, Long Yi-Tao
Key Laboratory for Advanced Materials, Department of Chemistry, East China University of Science and Technology, Shanghai, 200237, P. R. China.
Faraday Discuss. 2015;184:85-99. doi: 10.1039/c5fd00060b. Epub 2015 Sep 30.
Nanopore techniques have proven to be useful tools for single-molecule detection. The combination of optical detection and ionic current measurements enables a new possibility for the parallel readout of multiple nanopores without complex nanofluidics and embedded electrodes. In this study, we developed a new integrated system for the label-free optical and electrical detection of single molecules based on a metal-coated nanopore. The entire system, containing a dark-field microscopy system and an ultralow current detection system with high temporal resolution, was designed and fabricated. An Au-coated nanopore was used to generate the optical signal. Light scattering from a single Au-coated nanopore was measured under a dark-field microscope. A lab-built ultralow current detection system was designed for the correlated optical and electrical readout. This integrated system might provide more direct and detailed information on single analytes inside the nanopore compared with classical ionic current measurements.
纳米孔技术已被证明是用于单分子检测的有用工具。光学检测与离子电流测量的结合为无需复杂的纳米流体和嵌入式电极即可并行读取多个纳米孔提供了新的可能性。在本研究中，我们基于金属涂层纳米孔开发了一种用于单分子无标记光学和电学检测的新型集成系统。设计并制造了包含暗场显微镜系统和具有高时间分辨率的超低电流检测系统的整个系统。使用金涂层纳米孔来产生光信号。在暗场显微镜下测量单个金涂层纳米孔的光散射。设计了一个实验室构建的超低电流检测系统用于相关的光学和电学读取。与传统的离子电流测量相比，这个集成系统可能会提供关于纳米孔内单个分析物更直接和详细的信息。
