Benites R S R, Formagio A S N, Argandoña E J S, Volobuff C R F, Trevizan L N F, Vieira M C, Silva M S
Faculdade de Ciências Exatas e Tecnologia, Universidade Federal da Grande Dourados, Dourados, MS, BR.
Faculdade de Ciências Agrárias, Universidade Federal da Grande Dourados, Dourados, MS, BR.
Braz J Biol. 2015 Aug;75(3):685-91. doi: 10.1590/1519-6984.21313. Epub 2015 Sep 25.
The antioxidant potential of fruit pulp and seeds of extracts of the Annona coriacea, and A. sylvatica (Annonaceae) were investigated, as well contents total phenolics, flavonoids, condensed tannins and ascorbic acid. Was used to determine the antioxidant activity the 1,1-diphenyl-2-picrylhydrazyl free radical (DPPH), β-carotene bleaching and ABTS radical cation method. The total phenol, total flavonoid, condensed tannin, and ascorbic acid contents were measured spectrophotometrically. In this study, the pulp and seeds of the fruits were extracted using methanol/water (8:2) for maceration. The seed extracts of A. coriacea demonstrated a moderate antioxidant effect with free radical scavenging activity of 31.53%, by the DPPH test, 51.59% by the β-carotene bleaching test and 159.50 µM trolx/g of extract in the ABTS assay. We found that the hydromethanolic seed extract of A. coriacea had high total phenol (147.08 ± 4.20 mg of GAE/g of extract) and flavonoid (131.18 ± 2.31 mg of QE/g of extract) content. This indicated that the antioxidant activity of the extracts was related to the contents of these constituents.
对番荔枝科的糙叶番荔枝和林地番荔枝果实果肉及种子提取物的抗氧化潜力以及总酚、黄酮类化合物、缩合单宁和抗坏血酸的含量进行了研究。采用1,1 - 二苯基 - 2 - 苦基肼自由基(DPPH)法、β - 胡萝卜素漂白法和ABTS自由基阳离子法测定抗氧化活性。总酚、总黄酮、缩合单宁和抗坏血酸含量采用分光光度法测定。在本研究中,果实的果肉和种子用甲醇/水(8:2)进行浸渍提取。通过DPPH试验,糙叶番荔枝种子提取物表现出中等抗氧化作用,自由基清除活性为31.53%;通过β - 胡萝卜素漂白试验,自由基清除活性为51.59%;在ABTS试验中,提取物的抗氧化活性为159.50 μM Trolox/g。我们发现糙叶番荔枝种子的氢甲醇提取物具有较高的总酚(147.08 ± 4.20 mg没食子酸当量/g提取物)和黄酮类化合物(131.18 ± 2.31 mg槲皮素当量/g提取物)含量。这表明提取物的抗氧化活性与这些成分的含量有关。