一种具有多腔室的血管植入物，由涂有聚氧化乙烯的冬绿油/聚己内酯纤维电纺而成。
A multicompartment vascular implant of electrospun wintergreen oil/ polycaprolactone fibers coated with poly(ethylene oxide).
机构信息
Physics Department, Faculty of Science, Helwan University, Cairo, Egypt.
Polymers and Pigments Department, National Research Center, Cairo, Egypt.
出版信息
Biomed J. 2021 Oct;44(5):589-597. doi: 10.1016/j.bj.2020.04.008. Epub 2020 May 7.
BACKGROUND
The aim of the present study was to fabricate double layered scaffolds of electrospun polycaprolactone (PCL) and poly(ethylene oxide) (PEO). The electrospun PCL fibers were functionalized with wintergreen oil (WO) as a novel approach to prevent vascular grafts failure due to thrombosis by adjusting biomaterial-blood interactions.
METHODS
PCL tubular scaffolds were prepared by electrospinning approach and coated with PEO as a hydrophilic polymer. The single and double layered scaffolds were characterized in terms of their morphological, chemical properties -as well as-hemocompatibility assays (i.e. prothrombin time, hemolysis percentage and platelets adhesion). Moreover, the antioxidant potential of WO-PCL samples were measured by 2,2-diphenyl-1-picrylhydrazyl hydrate (DPPH) free radical assay.
RESULTS
The results demonstrated that incorporation of WO during the electrospinning process decreased the PCL fiber diameter. In addition, the prothrombine time assay shows that WO could be used to lower the electrospun PCL fiber tendency to induce blood clotting. Moreover, SEM observations of platelets adhesion of both single and double layered PCL/PEO scaffolds fiber shows an increase of platelets number, compared with the scaffolds containing WO.
CONCLUSIONS
The antioxidant potential and blood compatibility measurements of WO-PCL/PEO samples highlight the approach made so far as an ideal synthetic small size vascular grafts to overcome autogenous grafts shortages and drawbacks.
背景
本研究的目的是制备聚己内酯（PCL）和聚氧化乙烯（PEO）的双层支架。通过将水杨酸甲酯（WO）功能化到电纺 PCL 纤维上，作为一种调节生物材料与血液相互作用以防止血管移植物因血栓形成而失效的新方法。
方法
通过静电纺丝方法制备 PCL 管状支架，并涂覆亲水性聚合物 PEO。单层和双层支架在形态、化学性质以及血液相容性试验（即凝血酶原时间、溶血百分比和血小板黏附）方面进行了表征。此外，通过 2,2-二苯基-1-苦肼基肼（DPPH）自由基测定法测量 WO-PCL 样品的抗氧化潜力。
结果
结果表明，在静电纺丝过程中加入 WO 会降低 PCL 纤维的直径。此外，凝血酶原时间测定表明 WO 可用于降低电纺 PCL 纤维诱导血液凝结的趋势。此外，与含有 WO 的支架相比，SEM 观察到单层和双层 PCL/PEO 支架纤维上的血小板黏附数量增加。
结论
WO-PCL/PEO 样品的抗氧化潜力和血液相容性测量结果突出了迄今为止所采用的方法，即作为一种理想的合成小尺寸血管移植物，以克服自体移植物的短缺和缺点。
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