Gao Feng, Zhou Jingbin, He Chen, Ding Jie, Lou Zhikun, Xie Qiang, Li Haiwei, Li Fangxiang, Li Guoping
Department of Sports Injury and Arthroscopy Surgery, China National Institute of Sports Medicine, Beijing, China.
School of Physical Education, Shanxi Normal University, Linfen, China.
Arthroscopy. 2016 Feb;32(2):273-80. doi: 10.1016/j.arthro.2015.07.010. Epub 2015 Oct 1.
To investigate the morphology and quantity of mechanoreceptors in the remnant stumps of injured anterior cruciate ligaments (ACLs) and the association of the number of mechanoreceptors with the time from injury to surgery.
Complete ACL stump specimens were collected from 40 volunteer patients who underwent arthroscopic ACL reconstruction surgery. Serial sections, 20 μm in thickness, were prepared from each specimen. After H&E staining and immunohistochemical labeling, the morphology, type, size, and quantity of the mechanoreceptors were observed under an optical microscope.
A total of 176 Ruffini corpuscles, 61 pacinian corpuscles, 15 Golgi-like tendon organs, and 66 atypical mechanoreceptors were observed. Free nerve endings were commonly present in the specimens. The time from injury to surgery (log10 days) was negatively correlated with the number of total mechanoreceptors (r = -0.43, P < .006), Ruffini corpuscles (r = -0.45, P < .003), and the volume of the ACL stump (r = -0.52, P < .01), and these correlations were independent of age, gender, injury side, and career.
The number of mechanoreceptors in an ACL stump and the volume of the stump decreased with the time from injury to surgery.
This study provides a further theoretical basis for ACL reconstruction with remnant preservation.
研究前交叉韧带（ACL）损伤后残端中机械感受器的形态和数量，以及机械感受器数量与受伤至手术时间的关系。
从40例行关节镜下ACL重建手术的志愿者患者中采集完整的ACL残端标本。每个标本制备20μm厚的连续切片。经苏木精-伊红（H&E）染色和免疫组织化学标记后，在光学显微镜下观察机械感受器的形态、类型、大小和数量。
共观察到176个鲁菲尼小体、61个环层小体、15个高尔基腱器官样结构和66个非典型机械感受器。标本中常见游离神经末梢。受伤至手术时间（log10天）与机械感受器总数（r = -0.43，P <.006）、鲁菲尼小体数量（r = -0.45，P <.003）以及ACL残端体积（r = -0.52，P <.01）呈负相关，且这些相关性与年龄、性别、受伤侧和职业无关。
ACL残端中机械感受器的数量和残端体积随受伤至手术时间的延长而减少。
本研究为保留残端的ACL重建提供了进一步的理论依据。
