通过基质辅助激光解吸电离飞行时间质谱法对葡萄球菌属进行常规鉴定时评分截止值的优化
Optimization of the score cutoff value for routine identification of Staphylococcus species by matrix-assisted laser desorption ionization-time-of-flight mass spectrometry.
作者信息
Han Huan Wen, Chang Hsien Chang, Hunag Ay Huei, Chang Tsung Chain
机构信息
Institute of Biomedical Engineering, College of Engineering, National Cheng Kung University, Tainan, Taiwan.
Institute of Biomedical Engineering, College of Engineering, National Cheng Kung University, Tainan, Taiwan; Medical Device Innovation Center, National Cheng Kung University, Tainan, Taiwan.
出版信息
Diagn Microbiol Infect Dis. 2015 Dec;83(4):349-54. doi: 10.1016/j.diagmicrobio.2015.08.003. Epub 2015 Aug 7.
Staphylococcus species are important pathogens. We evaluated 2 score cutoffs (2.0 and 1.7) and the replicate number (a single or a duplicate test) on the identification of staphylococci using the Bruker matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS). A collection of 440 clinical isolates (11 species) and 144 reference strains (36 species) was evaluated. For clinical isolates using a cutoff of 2.0 and duplicate tests, the rates of species, genus, and unreliable identifications were 93.4%, 5.7%, and 0.9% respectively, while the respective values were 99.3%, 0.2%, and 0.5% when the cutoff was 1.7. The species identification rates were significantly higher (P<0.01) when a cutoff of 1.7 or a duplicate test was used. Similar results were obtained for reference strains. In conclusion, a cutoff of 1.7 and duplicate tests are recommended for identification of staphylococci using MALDI-TOF MS.
葡萄球菌属是重要的病原体。我们使用布鲁克基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）评估了2个评分截断值（2.0和1.7）以及重复次数（单次或重复测试）对葡萄球菌鉴定的影响。评估了440株临床分离株（11个种）和144株参考菌株（36个种）。对于临床分离株，使用截断值2.0并进行重复测试时，种、属和不可靠鉴定的比率分别为93.4%、5.7%和0.9%，而截断值为1.7时，相应的值分别为99.3%、0.2%和0.5%。使用截断值1.7或重复测试时，种鉴定率显著更高（P<0.01）。参考菌株也获得了类似结果。总之，推荐使用截断值1.7和重复测试通过MALDI-TOF MS鉴定葡萄球菌。
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