SiR-Hoechst是一种用于活细胞纳米显微镜检查的远红色DNA染色剂。
SiR-Hoechst is a far-red DNA stain for live-cell nanoscopy.
作者信息
Lukinavičius Gražvydas, Blaukopf Claudia, Pershagen Elias, Schena Alberto, Reymond Luc, Derivery Emmanuel, Gonzalez-Gaitan Marcos, D'Este Elisa, Hell Stefan W, Wolfram Gerlich Daniel, Johnsson Kai
机构信息
Ecole Polytechnique Fédérale de Lausanne (EPFL), Institute of Chemical Sciences and Engineering (ISIC), Institute of Bioengineering, NCCR in Chemical Biology, 1015 Lausanne, Switzerland.
Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna Biocenter Campus (VBC), Dr Bohr Gasse 3, 1030 Vienna, Austria.
出版信息
Nat Commun. 2015 Oct 1;6:8497. doi: 10.1038/ncomms9497.
Cell-permeable DNA stains are popular markers in live-cell imaging. Currently used DNA stains for live-cell imaging are either toxic, require illumination with blue light or are not compatible with super-resolution microscopy, thereby limiting their utility. Here we describe a far-red DNA stain, SiR-Hoechst, which displays minimal toxicity, is applicable in different cell types and tissues, and is compatible with super-resolution microscopy. The combination of these properties makes this probe a powerful tool for live-cell imaging.
细胞可渗透的DNA染料是活细胞成像中常用的标记物。目前用于活细胞成像的DNA染料要么有毒，需要蓝光照射，要么与超分辨率显微镜不兼容，从而限制了它们的实用性。在这里，我们描述了一种远红色DNA染料SiR-Hoechst，它显示出最小的毒性，适用于不同的细胞类型和组织，并且与超分辨率显微镜兼容。这些特性的结合使该探针成为活细胞成像的强大工具。
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