远红切换 DNA 探针用于活细胞纳米显微镜。
Far-red switching DNA probes for live cell nanoscopy.
机构信息
Chromatin Labeling and Imaging Group, Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany.
出版信息
Chem Commun (Camb). 2020 Nov 26;56(94):14797-14800. doi: 10.1039/d0cc06759h.
Herein we present DNA probes composed of Hoechst 33258 and spontaneously blinking far-red hydroxymethyl silicon-rhodamine (HMSiR). The best performing probe, 5-HMSiR-Hoechst, contains the 5'-regioisomer, shows ∼400-fold fluorescence increase upon DNA binding and is compatible with wash-free single molecule localization and 3D stimulated emission depletion microscopy of chromatin nanostructures in living cells.
在此，我们提出了由 Hoechst 33258 和自发闪烁的远红色羟甲基硅罗丹明（HMSiR）组成的 DNA 探针。表现最好的探针 5-HMSiR-Hoechst 包含 5'-位异构体，与染色质纳米结构在活细胞中的无洗涤单分子定位和 3D 受激发射损耗显微镜兼容，在与 DNA 结合时荧光增强约 400 倍。
Zotero 插件