用单丹磺酰戊烷对脂滴进行染色。

Staining of Lipid Droplets with Monodansylpentane.

作者信息

Chen Bo-Hua, Yang Huei-Jiun, Chou He-Yen, Chen Guang-Chao, Yang Wei Yuan

机构信息

Chemical Biology and Molecular Biophysics Program, Taiwan International Graduate Program, Taipei, Taiwan.

Institute of Biological Chemistry, Academia Sinica, 128 Academia Road Sec. 2, Nankang, Taipei, 115, Taiwan.

出版信息

Methods Mol Biol. 2017;1560:231-236. doi: 10.1007/978-1-4939-6788-9_17.

DOI:10.1007/978-1-4939-6788-9_17

PMID:28155158

Abstract

Monodansylpentane (MDH) is a fluorophore that displays selective labeling of lipid droplets (LDs). The dye preferentially segregates into the neutral lipid cores of LDs and emits blue fluorescence, compatible with the simultaneous use of green and red fluorescent reporters in multi-color live-cell imaging. MDH can be used for visualizing LDs not only in cell cultures, but also in fixed tissues such as the fat body and ovaries from Drosophila. MDH is therefore a versatile marker for LDs in fluorescence microscopy.

摘要

单丹磺酰戊烷（MDH）是一种荧光团，可对脂滴（LDs）进行选择性标记。该染料优先分隔到脂滴的中性脂质核心中并发出蓝色荧光，这与在多色活细胞成像中同时使用绿色和红色荧光报告分子兼容。MDH不仅可用于在细胞培养物中可视化脂滴，还可用于在固定组织（如果蝇的脂肪体和卵巢）中可视化脂滴。因此，MDH是荧光显微镜中脂滴的一种通用标记物。