利用CRISPR/Cas9系统在秀丽隐杆线虫中创建基因组修饰

Creating Genome Modifications in C. elegans Using the CRISPR/Cas9 System.

作者信息

Calarco John A, Friedland Ari E

机构信息

FAS Center for Systems Biology, Harvard University, Northwest Lab Building, 52 Oxford Street, B227.80, Cambridge, MA, 02138, USA.

Editas Medicine, Cambridge, MA, USA.

出版信息

Methods Mol Biol. 2015;1327:59-74. doi: 10.1007/978-1-4939-2842-2_6.

DOI:10.1007/978-1-4939-2842-2_6

PMID:26423968

Abstract

The clustered, regularly interspaced, short, palindromic repeat (CRISPR)-associated (CAS) nuclease Cas9 has been used in many organisms to generate specific mutations and transgene insertions. Here we describe a method using the S. pyogenes Cas9 in C. elegans that provides a convenient and effective approach for making heritable changes to the worm genome.

摘要

成簇规律间隔短回文重复序列（CRISPR）相关的（CAS）核酸酶Cas9已被用于许多生物体中以产生特定突变和转基因插入。在此，我们描述了一种在秀丽隐杆线虫中使用化脓性链球菌Cas9的方法，该方法为对线虫基因组进行可遗传改变提供了一种方便有效的途径。