秀丽隐杆线虫中 CRISPR/Cas9 基因组编辑的方法。

Approaches for CRISPR/Cas9 Genome Editing in C. elegans.

机构信息

Department of Cell and Systems Biology, University of Toronto, Toronto, ON, Canada.

出版信息

Methods Mol Biol. 2022;2468:215-237. doi: 10.1007/978-1-0716-2181-3_11.

PMID:35320567

Abstract

The clustered, regularly interspaced, short, palindromic repeat (CRISPR)-associated (CAS) nuclease Cas9 has been used in many organisms to generate specific mutations and transgene insertions. Here we describe our most up-to-date protocols using the S. pyogenes Cas9 in C. elegans that provides a convenient and effective approach for making heritable changes to the worm genome. We present several considerations when deciding which strategy best suits the needs of the experiment.

摘要

成簇、规律间隔、短回文重复序列（CRISPR）相关（CAS）核酸酶 Cas9 已被用于许多生物中，以产生特定的突变和转基因插入。在此，我们描述了在秀丽隐杆线虫中使用 S. pyogenes Cas9 的最新方案，为对蠕虫基因组进行可遗传的改变提供了一种方便有效的方法。我们提出了一些需要考虑的因素，以确定哪种策略最适合实验的需求。