观察和量化荧光报告分子。

Observing and Quantifying Fluorescent Reporters.

作者信息

Hendricks Michael

机构信息

Department of Biology, McGill University, 1205 Ave Docteur Penfield, Rm N5/12, Montreal, QC, Canada, H3A 1B1.

出版信息

Methods Mol Biol. 2015;1327:75-85. doi: 10.1007/978-1-4939-2842-2_7.

DOI:10.1007/978-1-4939-2842-2_7

PMID:26423969

Abstract

Genetically encoded fluorescent reporters take advantage of C. elegans' transparency to allow noninvasive, in vivo observation and recording of physiological processes in intact animals. Here, I discuss the basic microscope components required to observe, image, and measure fluorescent proteins in live animals for students and researchers who work with C. elegans but have limited experience with fluorescence imaging and analysis.

摘要

基因编码荧光报告分子利用秀丽隐杆线虫的透明性，实现对完整动物体内生理过程的非侵入性观察和记录。在此，我将为从事秀丽隐杆线虫研究但在荧光成像和分析方面经验有限的学生和研究人员，讨论在活体动物中观察、成像和测量荧光蛋白所需的基本显微镜组件。