Zhang Jiaojiao, Ma Guannan, Deng Yuangao, Dong Jinggang, Van Stappen Gilbert, Sui Liying
Tianjin Key Laboratory of Marine Resources and Chemistry, College of Marine and Environmental Science, Tianjin University of Science and Technology, Tianjin, 300457, China.
Laboratory of Aquaculture and Artemia Reference Center, Ghent University, 9000, Ghent, Belgium.
Curr Microbiol. 2016 Jan;72(1):55-63. doi: 10.1007/s00284-015-0916-5. Epub 2015 Sep 30.
The microbiota in solar salterns plays an important role in salt production quantitatively and qualitatively. Bohai Bay coast is the major sea salt producing area in China. However, few ecological characterization studies of the Bohai Bay salt ponds, particularly of their microbial diversity, have been conducted. This study investigated the structure and diversity of the bacterial community in Hangu saltworks in response to environmental factors. The brine water was sampled from five selected saltponds within a salinity range of 5.0-19.3% in May, July, and October, 2012. Phylogenetic analysis based on the denaturing gradient gel electrophoresis (DGGE) patterns of the PCR-amplified 16S rRNA gene fragment showed that, rather than pond salinity, especially the month of sampling influenced the structure of the bacterial community in the saltponds, which may be related to the water temperature or other factors fluctuating over the months. Moreover, canonical correspondence analysis of biological and physico-chemical parameters indicated that especially other environmental factors such as nitrogenous and phosphorous nutrient contents and pH structured the microbial community. The relatively high range-weighted richness index and Shannon-Wiener index (H') observed in this study reflect the high level of richness and biodiversity present, though there were substantial fluctuations over the months and salinities of sampling. The fragment of 16S rRNA gene sequence recovered from DGGE bands indicated that the bacterial assemblage in Hangu Saltworks was dominated by members of γ-Proteobacteria (34% of total sequences obtained), followed by Firmicutes (14%) and Bacteroidetes (9%).
太阳能盐场中的微生物群落在盐的生产过程中，无论在数量上还是质量上都发挥着重要作用。渤海湾沿岸是中国主要的海盐产区。然而，针对渤海湾盐池，尤其是其微生物多样性的生态特征研究却很少。本研究调查了汉沽盐场细菌群落的结构和多样性，以及环境因素对其的影响。于2012年5月、7月和10月，从五个选定的盐池中采集盐水样本，盐度范围为5.0-19.3%。基于PCR扩增的16S rRNA基因片段的变性梯度凝胶电泳（DGGE）图谱进行系统发育分析，结果表明，影响盐池细菌群落结构的并非盐池盐度，尤其是采样月份，这可能与水温或其他随月份波动的因素有关。此外，对生物和理化参数的典范对应分析表明，尤其是其他环境因素，如氮磷营养成分和pH值，构建了微生物群落。尽管在采样月份和盐度上存在大幅波动，但本研究中观察到的相对较高的范围加权丰富度指数和香农-威纳指数（H'）反映了所存在的丰富度和生物多样性的高水平。从DGGE条带中回收的16S rRNA基因序列片段表明，汉沽盐场的细菌组合以γ-变形菌门成员为主（占获得的总序列的34%），其次是厚壁菌门（14%）和拟杆菌门（9%）。
