Hu Y, Liu X, Qiao D
College of Physical Education and Sports, Beijing Normal University, Beijing, 100875, China ; Department of Physical Education of Central South University, Changsha Hunan, 410083, China.
College of Physical Education and Sports, Beijing Normal University, Beijing, 100875, China.
Biol Sport. 2015 Sep;32(3):187-92. doi: 10.5604/20831862.1150299. Epub 2015 Apr 24.
The purpose of the study was to explore the mechanism underlying the enhanced subthalamic nucleus (STN) neural activity during exhausting exercise from the perspective of monoamine neurotransmitters and changes of their corresponding receptors. Rats were randomly divided into microdialysis and immunohistochemistry study groups. For microdialysis study, extracellular fluid of the STN was continuously collected with a microdialysis probe before, during and 90 min after one bout of exhausting exercise. Dopamine (DA) and 5-hydroxytryptamine (5-HT) levels were subsequently detected with high-performance liquid chromatography (HPLC). For immunohistochemistry study, the expression of DRD2 and HT2C receptors in the STN, before, immediately after and 90 min after exhaustion was detected through immunohistochemistry technique. Microdialysis study results showed that the extracellular DA and 5-HT neurotransmitters increased significantly throughout the procedure of exhausting exercise and the recovery period (P<0.05 or P<0.01). Immunohistochemistry study results showed that the expression levels of DRD2 and HT2C in the rat STN immediately after exhausting exercise and at the time point of 90 min after exhaustion were both higher than those of the rest condition, but the difference was not significant (P>0.05). Our results suggest that the increased extracellular DA and 5-HT in the STN might be one important factor leading to the enhanced STN neural activity and the development of fatigue during exhausting exercise. This study may essentially offer useful evidence for better understanding of the mechanism of the central type of exercise-induced fatigue.
本研究的目的是从单胺类神经递质及其相应受体变化的角度，探讨力竭运动过程中丘脑底核（STN）神经活动增强的机制。将大鼠随机分为微透析和免疫组化研究组。对于微透析研究，在一次力竭运动前、运动期间和运动后90分钟，用微透析探针连续收集STN的细胞外液。随后用高效液相色谱法（HPLC）检测多巴胺（DA）和5-羟色胺（5-HT）水平。对于免疫组化研究，通过免疫组化技术检测力竭前、力竭后即刻和力竭后90分钟时STN中DRD2和HT2C受体的表达。微透析研究结果显示，在力竭运动及恢复期，细胞外DA和5-HT神经递质均显著增加（P<0.05或P<0.01）。免疫组化研究结果显示，力竭后即刻及力竭后90分钟时大鼠STN中DRD2和HT2C的表达水平均高于其余状态，但差异不显著（P>0.05）。我们的结果表明，STN中细胞外DA和5-HT的增加可能是导致力竭运动期间STN神经活动增强及疲劳发生的一个重要因素。本研究可能从本质上为更好地理解中枢性运动性疲劳的机制提供有用的证据。
