Datta Suvro Sankha, Mukherjee Somnath, Talukder Biplabendu, Bhattacharya Prasun, Mukherjee Krishnendu
Department of Transfusion Medicine, The Mission Hospital, Durgapur, West Bengal 713212, India.
Department of Transfusion Medicine, AIIMS, Bhubaneswar 751019, India.
Adv Hematol. 2015;2015:610931. doi: 10.1155/2015/610931. Epub 2015 Sep 6.
Introduction. Red blood cell (RBC) alloimmunization and autoimmunization remain a major problem in transfusion dependent thalassemic patients. There is a paucity of data on the incidence of RBC alloimmunization and autoimmunization in thalassemic patients from eastern part of India, as pretransfusion antibody screening is not routinely performed. Aims. To assess the incidence of RBC alloimmunization and autoimmunization in transfusion dependent thalassemic patients in eastern India. Materials and Methods. Total 500 thalassemia cases were evaluated. The antibody screening and identification were performed with commercially available panel cells (Diapanel, Bio-rad, Switzerland) by column agglutination method. To detect autoantibodies, autocontrol and direct antiglobulin tests were carried out using polyspecific coombs (IgG + C3d) gel cards in all patients. Results. A total of 28 patients developed RBC alloimmunization (5.6%) and 5 patients had autoantibodies (1%). Alloantibody against c had the highest incidence (28.57%) followed by E (21.42%). Five out of 28 (17.85%) patients had developed antibodies against both c and E. Conclusion. Data from this study demonstrate that the RBC alloantibody and autoantibody development rates are significant in our region. Thus, pretransfusion antibody screening needs to be initiated in eastern India in order to ensure safe transfusion practice.
引言。红细胞（RBC）同种免疫和自身免疫仍是依赖输血的地中海贫血患者面临的一个主要问题。由于未常规进行输血前抗体筛查，关于印度东部地中海贫血患者红细胞同种免疫和自身免疫发生率的数据匮乏。目的。评估印度东部依赖输血的地中海贫血患者红细胞同种免疫和自身免疫的发生率。材料与方法。共评估了500例地中海贫血病例。采用柱凝集法，使用市售的细胞面板（DiaPanel，伯乐公司，瑞士）进行抗体筛查和鉴定。为检测自身抗体，对所有患者使用多特异性抗人球蛋白（IgG + C3d）凝胶卡进行自身对照和直接抗人球蛋白试验。结果。共有28例患者发生红细胞同种免疫（5.6%），5例患者有自身抗体（1%）。抗c同种抗体的发生率最高（28.57%），其次是抗E（21.42%）。28例患者中有5例（17.85%）同时产生了抗c和抗E抗体。结论。本研究数据表明，在我们地区红细胞同种抗体和自身抗体的产生率较高。因此，印度东部需要开展输血前抗体筛查，以确保安全的输血操作。
