Yang X Y, Gasparro F P, DeLeo V A, Santella R M
Comprehensive Cancer Center, Columbia University, New York 10032.
J Invest Dermatol. 1989 Jan;92(1):59-63. doi: 10.1111/1523-1747.ep13071224.
The combination of 8-methoxypsoralen (8-MOP) plus ultraviolet A light (320-400 nm), termed PUVA, is used in the treatment of psoriasis, a hyperproliferative disease of the skin. This treatment results in the formation of specific 8-MOP adducts with cellular DNA. We have previously developed monoclonal antibodies which recognize these 8-MOP photoadducts. We now report the use of these antibodies in an indirect immunofluorescence technique to study human skin biopsies. Nuclei in 3 of 5 skin biopsies from psoriasis patients undergoing PUVA therapy were positive for adducts. The presence of adducts by immunofluorescence did not correlate with plasma levels of 8-MOP. Enzyme-linked immunosorbent assays, used to determine whether 8-MOP photoadducts could be detected in DNA isolated from the lymphocytes of psoriasis patients after PUVA therapy, were negative.
8-甲氧基补骨脂素（8-MOP）与紫外线A光（320 - 400纳米）联合使用，即PUVA疗法，用于治疗银屑病，一种皮肤过度增殖性疾病。这种治疗会导致8-MOP与细胞DNA形成特定加合物。我们之前已开发出可识别这些8-MOP光加合物的单克隆抗体。我们现在报告这些抗体在间接免疫荧光技术中用于研究人类皮肤活检样本的情况。接受PUVA治疗的银屑病患者的5份皮肤活检样本中有3份的细胞核加合物呈阳性。通过免疫荧光检测到的加合物的存在与8-MOP的血浆水平无关。用于确定PUVA治疗后银屑病患者淋巴细胞分离出的DNA中是否能检测到8-MOP光加合物的酶联免疫吸附测定结果为阴性。
