Kovacs J A, Halpern J L, Lundgren B, Swan J C, Parrillo J E, Masur H
Critical Care Medicine Department, National Institutes of Health, Bethesda, Maryland 20892.
J Infect Dis. 1989 Jan;159(1):60-70. doi: 10.1093/infdis/159.1.60.
To increase understanding of the antigenic structure of Pneumocystis carinii, we developed monoclonal antibodies to rat and human P. carinii. The specificity of the antibodies was demonstrated by immunofluorescence and immunoblot studies. Only one of five monoclonal antibodies to rat P. carinii reacted with human P. carinii, and none of four monoclonal antibodies to human P. carinii reacted with rat P. carinii. Two antibodies to human P. carinii reacted by immunofluorescence with only one human P. carinii isolate. Immunoblot studies identified major antigens of rat P. carinii with molecular masses of 40,000-100,000 daltons and of human P. carinii with molecular masses of 22,000-95,000 daltons. These studies document the existence of antigenic differences between rat and human P. carinii and are consistent with the suggestion that individual isolates of human P. carinii are also antigenically different. Further studies with these antibodies should increase understanding of the antigenic nature of P. carinii and of the interaction of P. carinii with its host.
为了增进对卡氏肺孢子虫抗原结构的了解，我们制备了针对大鼠和人卡氏肺孢子虫的单克隆抗体。通过免疫荧光和免疫印迹研究证实了这些抗体的特异性。针对大鼠卡氏肺孢子虫的五种单克隆抗体中只有一种与人类卡氏肺孢子虫发生反应，而针对人类卡氏肺孢子虫的四种单克隆抗体均未与大鼠卡氏肺孢子虫发生反应。两种针对人类卡氏肺孢子虫的抗体通过免疫荧光仅与一种人类卡氏肺孢子虫分离株发生反应。免疫印迹研究确定了大鼠卡氏肺孢子虫分子量为40,000 - 100,000道尔顿的主要抗原以及人类卡氏肺孢子虫分子量为22,000 - 95,000道尔顿的主要抗原。这些研究证明了大鼠和人类卡氏肺孢子虫之间存在抗原差异，并且与人类卡氏肺孢子虫的各个分离株在抗原性上也存在差异这一观点一致。使用这些抗体进行的进一步研究应能增进对卡氏肺孢子虫抗原性质以及卡氏肺孢子虫与其宿主相互作用的了解。
