Jones Jace W, Tudor Gregory, Li Fei, Tong Yan, Katz Barry, Farese Ann M, MacVittie Thomas J, Booth Catherine, Kane Maureen A
*University of Maryland, School of Pharmacy, Department of Pharmaceutical Sciences, Baltimore, MD; †Epistem Ltd, Manchester, UK; ‡Indiana University, School of Medicine and Richard M. Fairbanks School of Public Health, Department of Biostatistics, Indianapolis, IN; §University of Maryland, School of Medicine, Department of Radiation Oncology, Baltimore, MD.
Health Phys. 2015 Nov;109(5):452-65. doi: 10.1097/HP.0000000000000346.
The use of plasma citrulline as a biomarker for gastrointestinal acute radiation syndrome via exposure to total-body irradiation in a murine model was investigated. The radiation exposure covered lethal, mid-lethal, and sub-lethal gastrointestinal acute radiation syndrome. Plasma citrulline profiles were generated over the first 6 d following total-body irradiation exposure of 6-15 Gy. In addition, plasma citrulline was comprehensively evaluated in the context of matching small intestine citrulline and histopathology. Higher plasma citrulline was significantly associated with lower irradiation doses over the first 6 d following the irradiation insult. Furthermore, higher plasma citrulline was significantly associated with higher crypt survival. The correlation of the plasma citrulline to crypt survival was more robust for higher irradiation doses and for later time points. The data suggested plasma citrulline was most informative for reflecting gastrointestinal injury resulting from exposure to 9-15 Gy total-body irradiation covering time-points 2-5 d post the irradiation insult.
研究了在小鼠模型中，通过全身照射将血浆瓜氨酸用作胃肠道急性放射综合征生物标志物的情况。辐射暴露涵盖致死性、中度致死性和亚致死性胃肠道急性放射综合征。在6-15 Gy全身照射后的前6天内生成血浆瓜氨酸谱。此外，还结合匹配的小肠瓜氨酸和组织病理学对血浆瓜氨酸进行了全面评估。在照射损伤后的前6天，较高的血浆瓜氨酸与较低的照射剂量显著相关。此外，较高的血浆瓜氨酸与较高的隐窝存活率显著相关。对于较高的照射剂量和较晚的时间点，血浆瓜氨酸与隐窝存活率的相关性更强。数据表明，血浆瓜氨酸对于反映暴露于9-15 Gy全身照射后2-5天时间点的胃肠道损伤最具信息量。
