Prudencio Almudena, Faig Jonathan J, Song MinJung, Uhrich Kathryn E
Department of Chemistry and Chemical Biology, Rutgers University, 610 Taylor Road, Piscataway, NJ 08854-8087, USA.
Department of Biomedical Engineering, Rutgers University, 599 Taylor Road, Piscataway, NJ 08854-8087, USA.
Macromol Biosci. 2016 Feb;16(2):214-22. doi: 10.1002/mabi.201500244. Epub 2015 Oct 1.
Poly(anhydride-esters) comprised of naturally occurring, non-toxic phenolic acids, namely syringic and vanillic acid, with antioxidant properties were prepared via solution polymerization methods. Polymer and polymer precursor physiochemical properties were characterized, including polymer molecular weight and thermal properties. In vitro release studies illustrated that polymer hydrolytic degradation was influenced by relative hydrophobicity and degree of methoxy substitution of the phenolic acids. Further, the released phenolic acids were found to maintain antioxidant potency relative to free phenolic acid controls as determined by a 2,2-diphenyl-1-picrylhydrazyl assay. Polymer cytotoxicity was assessed with L929 fibroblasts in polymer-containing media; appropriate cell morphology and high fibroblast proliferation were obtained for the polymers at the lower concentrations. These polymers deliver non-cytotoxic levels of naturally occurring antioxidants, which could be efficacious in topical delivery of antioxidant therapies.
通过溶液聚合法制备了由天然存在的无毒酚酸（即丁香酸和香草酸）组成的具有抗氧化性能的聚（酸酐 - 酯）。对聚合物和聚合物前体的物理化学性质进行了表征，包括聚合物分子量和热性质。体外释放研究表明，聚合物的水解降解受酚酸相对疏水性和甲氧基取代程度的影响。此外，通过2,2 - 二苯基 - 1 - 苦基肼测定法确定，释放出的酚酸相对于游离酚酸对照物保持抗氧化效力。在含聚合物的培养基中用L929成纤维细胞评估聚合物的细胞毒性；较低浓度的聚合物获得了适当的细胞形态和高成纤维细胞增殖率。这些聚合物可递送天然抗氧化剂的非细胞毒性水平，这在抗氧化疗法的局部递送中可能是有效的。
