Paiva Thiago da Silva, Shao Chen, Fernandes Noemi Mendes, Borges Bárbara do Nascimento, da Silva-Neto Inácio Domingos
Laboratório de Protistologia, Departamento de Zoologia, Instituto de Biologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro - RJ, Brazil.
Laboratório de Biologia Molecular "Francisco Mauro Salzano", Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, Pará, Brazil.
J Eukaryot Microbiol. 2016 Mar-Apr;63(2):247-61. doi: 10.1111/jeu.12273. Epub 2015 Oct 28.
Interphase specimens, aspects of physiological reorganization and divisional morphogenesis were investigated in a strain of a hypotrichous ciliate highly similar to Urostyla grandis Ehrenberg, (type species of Urostyla), collected from a mangrove area in the estuary of the Paraíba do Sul river (Rio de Janeiro, Brazil). The results revealed that albeit interphase specimens match with the known morphologic variability in U. grandis, morphogenetic processes have conspicuous differences. Parental adoral zone is entirely renewed during morphogenesis, and marginal cirri exhibit a unique combination of developmental modes, in which left marginal rows originate from multiple anlagen arising from innermost left marginal cirral row, whereas right marginal ciliature originates from individual within-row anlagen. Based on such characteristics, a new subspecies, namely U. grandis wiackowskii subsp. nov. is proposed, and consequently, U. grandis grandis Ehrenberg, stat. nov. is established. Bayesian and maximum-likelihood analyses of the 18S rDNA unambiguously placed U. grandis wiackowskii as adelphotaxon of a cluster formed by other U. grandis sequences. The implications of such findings to the systematics of Urostyla are discussed.
对一种与大尾柱虫（尾柱虫属的模式种）高度相似的腹毛目纤毛虫菌株进行了研究，该菌株采自巴西里约热内卢帕拉伊巴杜斯尔河河口的红树林地区，研究内容包括间期标本、生理重组和分裂形态发生的各个方面。结果表明，尽管间期标本与大尾柱虫已知的形态变异性相符，但形态发生过程存在明显差异。在形态发生过程中，亲代口前区会完全更新，边缘纤毛呈现出独特的发育模式组合，其中左边缘列起源于最内侧左边缘纤毛列产生的多个原基，而右边缘纤毛结构则起源于行内单个原基。基于这些特征，提出了一个新亚种，即大尾柱虫维阿科夫斯基亚种（U. grandis wiackowskii subsp. nov.），因此，建立了大尾柱虫指名亚种（U. grandis grandis Ehrenberg, stat. nov.）。对18S rDNA进行的贝叶斯分析和最大似然分析明确地将大尾柱虫维阿科夫斯基亚种定位为一个由其他大尾柱虫序列形成的簇的姐妹分类单元。讨论了这些发现对尾柱虫系统学的意义。
