Department of Biology, Gangneung-Wonju National University, Jukheon-gil 7, Gangneung, Gangwon-do, 25457, South Korea.
Natural Science Research Institute, Gangneung-Wonju National University, Gangneung, 25457, South Korea.
J Eukaryot Microbiol. 2020 Jan;67(1):54-65. doi: 10.1111/jeu.12753. Epub 2019 Aug 18.
The morphology and morphogenesis of a new ciliate species, Neobakuella aenigmatica n. sp., which was discovered in an estuary in Korea, were investigated, using live observation, protargol impregnation, and scanning electron microscopy. This new species is characterized by a large (185-300 × 55-105 μm in vivo), elongate-ellipsoidal, flexible but not contractile body. It has ellipsoidal, yellowish cortical granules, 1.3 × 1.0 μm in size. The species has invariably 3 frontal and 2 frontoterminal cirri, about 5-10 buccal and 1-6 parabuccal cirri, 7 midventral rows, and 1 right and 2-4 left marginal rows. The outer left marginal row(s) consists of 1-7 short rows of cirri. The nuclear apparatus comprises 130 macronuclear nodules and 2 spherical micronuclei on average. The dorsal ciliature consists of 3 dorsal kineties. The leftmost left marginal row(s) likely develops from anlagen originating from both the rightmost and leftmost left marginal row(s). The molecular phylogenetic tree based on SSU rDNA suggests the nonmonophyly of the genus Neobakuella.
采用活体观察、蛋白银浸染和扫描电子显微镜技术，对在韩国一个河口发现的一种新纤毛虫物种，即 Neobakuella aenigmatica n. sp. 的形态和形态发生进行了研究。该新种的特征为大型（活体时为 185-300×55-105μm）、长椭圆形、柔软但不收缩的身体。它具有椭圆形、略带黄色的皮层颗粒，大小为 1.3×1.0μm。该物种始终具有 3 个额前纤毛和 2 个额前顶纤毛，约有 5-10 个口纤毛和 1-6 个副口纤毛，7 条中腹纤毛列和 1 条右缘纤毛列和 2-4 条左缘纤毛列。外左缘纤毛列由 1-7 个短纤毛列组成。核器包含 130 个大核小体和 2 个平均大小的球形微核。背纤毛由 3 个背动基体组成。最左侧的左缘纤毛列（多个）可能由最右侧和最左侧的左缘纤毛列（多个）的原基发育而来。基于 SSU rDNA 的分子系统发育树表明 Neobakuella 属的非单系性。
