Jiang Xuhong, Lv Bin, Shen Qianhong, Wang Xinmin
The First Clinical Medical College of Zhejiang Chinese Medical University, Hangzhou, China.
Emergency Department, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, China.
J Biomed Mater Res B Appl Biomater. 2017 Jan;105(1):91-98. doi: 10.1002/jbm.b.33530. Epub 2015 Oct 1.
Antimicrobial coating of polyethylene endotracheal tubes (PE ETTs) has proven to be an effective method to prevent endoluminal biofilm formation. A transparent silicon-modified antimicrobial PE ETT was obtained by coating PE with a SiO /γ-methacryloxypropyl trimethoxy silane (KH-570)/methyltriethoxysilane (MTES)/Ag-SiO solution prepared by chemically mixing Ag-SiO with SiO /KH-570/MTES in solution via a dip-coating method, with tetraethyl orthosilicate (TEOS) as the inorganic silicon source, followed by drying. All the films were characterized by various techniques, including the pencil hardness test, infrared spectroscopy, scanning electron microscopy, UV-vis analysis, and inductively coupled plasma mass spectrometry (ICP-MS). The results indicated that the TEOS/KH-570/MTES/Ag-SiO (15:6:1:0.6-1.0) films, which exhibited simple in-solution film formation on PE ETTs, had a homogeneous morphology, high transmittance above 87%, high hardness of 5H and strong adhesion to the tubes. The concentration of Ag ion dissolved out from the antibacterial coating is very low in ICP-MS results. The antibacterial test results show that the antibacterial coatings have excellent antibacterial property with antibacterial ratio up to 93.5% when Ag-SiO content is 2.6%. In pyrogen test and hemolytic test, the body temperature of rabbits rise 0.03°c for 3 h after inserting antibacterial PE ETT, and the hemolytic ratio is 0.7512%, which conform to the requirements of biomedical material. The results preliminarily proved that the antibacterial materials could be a good candidate of medical catheter material application or medical device surface coating materials. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 91-98, 2017.
事实证明，聚乙烯气管内导管（PE ETTs）的抗菌涂层是防止管腔内生物膜形成的有效方法。通过浸涂法，用通过将Ag-SiO与SiO /KH-570/MTES在溶液中化学混合制备的SiO /γ-甲基丙烯酰氧基丙基三甲氧基硅烷（KH-570）/甲基三乙氧基硅烷（MTES）/Ag-SiO溶液涂覆PE，以正硅酸乙酯（TEOS）作为无机硅源，然后干燥，从而获得透明的硅改性抗菌PE ETT。所有薄膜均通过各种技术进行表征，包括铅笔硬度测试、红外光谱、扫描电子显微镜、紫外可见分析和电感耦合等离子体质谱（ICP-MS）。结果表明，TEOS/KH-570/MTES/Ag-SiO（15:6:1:0.6 - 1.0）薄膜在PE ETTs上表现出简单的溶液成膜，具有均匀的形态、87%以上的高透光率、5H的高硬度以及与导管的强附着力。ICP-MS结果显示，从抗菌涂层中溶出的Ag离子浓度非常低。抗菌测试结果表明，当Ag-SiO含量为2.6%时，抗菌涂层具有优异的抗菌性能，抗菌率高达93.5%。在热原测试和溶血测试中，插入抗菌PE ETT后3小时，兔子体温升高0.03°C，溶血率为0.7512%，符合生物医学材料的要求。结果初步证明，该抗菌材料可能是医用导管材料应用或医疗器械表面涂层材料的良好候选者。© 2015威利期刊公司。《生物医学材料研究杂志》B部分：应用生物材料，105B：91 - 98，2017年。
