Cazalini Elisa M, Miyakawa Walter, Teodoro Guilherme R, Sobrinho Argemiro S S, Matieli José E, Massi Marcos, Koga-Ito Cristiane Y
Department of Physics, Technological Institute of Aeronautics - ITA, São José dos Campos, SP, Brazil.
Oral Biopathology Graduate Program, Institute of Science and Technology, São Paulo State University - UNESP, São José dos Campos, SP, Brazil.
J Mater Sci Mater Med. 2017 Jun;28(6):97. doi: 10.1007/s10856-017-5910-y. Epub 2017 May 17.
A promising strategy to reduce nosocomial infections related to prosthetic meshes is the prevention of microbial colonization. To this aim, prosthetic meshes coated with antimicrobial thin films are proposed. Commercial polypropylene meshes were coated with metal-containing diamond-like carbon (Me-DLC) thin films by the magnetron sputtering technique. Several dissimilar metals (silver, cobalt, indium, tungsten, tin, aluminum, chromium, zinc, manganese, tantalum, and titanium) were tested and compositional analyses of each Me-DLC were performed by Rutherford backscattering spectrometry. Antimicrobial activities of the films against five microbial species (Candida albicans, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Enterococcus faecalis) were also investigated by a modified Kirby-Bauer test. Results showed that films containing silver and cobalt have inhibited the growth of all microbial species. Tungsten-DLC, tin-DLC, aluminum-DLC, zinc-DLC, manganese-DLC, and tantalum-DLC inhibited the growth of some strains, while chromium- and titanium-DLC weakly inhibited the growth of only one tested strain. In-DLC film showed no antimicrobial activity. The effects of tungsten-DLC and cobalt-DLC on Pseudomonas aeruginosa biofilm formation were also assessed. Tungsten-DLC was able to significantly reduce biofilm formation. Overall, the experimental results in the present study have shown new approaches to coating polymeric biomaterials aiming antimicrobial effect.
减少与人工合成补片相关的医院感染的一个有前景的策略是预防微生物定植。为此，有人提出了涂覆有抗菌薄膜的人工合成补片。通过磁控溅射技术在商用聚丙烯补片上涂覆含金属的类金刚石碳（Me-DLC）薄膜。测试了几种不同的金属（银、钴、铟、钨、锡、铝、铬、锌、锰、钽和钛），并通过卢瑟福背散射光谱法对每种Me-DLC进行了成分分析。还通过改良的 Kirby-Bauer 试验研究了这些薄膜对五种微生物（白色念珠菌、大肠杆菌、铜绿假单胞菌、金黄色葡萄球菌和粪肠球菌）的抗菌活性。结果表明，含银和钴的薄膜抑制了所有微生物的生长。钨-DLC、锡-DLC、铝-DLC、锌-DLC、锰-DLC 和钽-DLC 抑制了一些菌株的生长，而铬-DLC 和钛-DLC 仅对一种受试菌株的生长有微弱抑制作用。铟-DLC 薄膜没有抗菌活性。还评估了钨-DLC 和钴-DLC 对铜绿假单胞菌生物膜形成的影响。钨-DLC 能够显著减少生物膜的形成。总体而言，本研究中的实验结果展示了针对抗菌效果对聚合物生物材料进行涂层的新方法。
