Hendil-Forssell Peter, Martinelle Mats, Syrén Per-Olof
KTH Royal Institute of Technology, Division of Industrial Biotechnology, AlbaNova University Centre, 106 91 Stockholm, Sweden.
KTH Royal Institute of Technology, Division of Proteomics & Nanobiotechnology, Science for Life Laboratory, 171 21 Stockholm, Sweden.
Chem Commun (Camb). 2015 Dec 18;51(97):17221-4. doi: 10.1039/c5cc07162c.
A novel enzyme engineering strategy for accelerated catalysis based on redesigning a water network through protein backbone deshielding is presented. Fundamental insight into the energetic consequences associated with the design is discussed in the light of experimental results and computer simulations. Using water as biobricks provides unique opportunities when transition state stabilisation is not easily attained by traditional enzyme engineering.
本文提出了一种基于通过蛋白质主链去屏蔽重新设计水网络来加速催化的新型酶工程策略。根据实验结果和计算机模拟，讨论了对与该设计相关的能量后果的基本见解。当传统酶工程难以实现过渡态稳定时，利用水作为生物构件提供了独特的机会。
