Müller Norbert, Hentrich Brigitte, Frey Caroline F, Welle Monika
Institute of Parasitology, Vetsuisse Faculty, University of Berne, Berne, Switzerland.
Institute of Parasitology, Vetsuisse Faculty, University of Berne, Berne, Switzerland.
Mol Cell Probes. 2015 Dec;29(6):507-510. doi: 10.1016/j.mcp.2015.09.008. Epub 2015 Sep 30.
The present report describes a real-time PCR-based procedure to reliably determine the quantity of Leishmania amastigotes in relation to the amount of host tissue in histological skin sections from canine and equine cases of cutaneous leishmaniasis. The novel diagnostic Leishmania-PCR has a detection limit of <0.02 amastigotes per μg tissue, which corresponds well to the detection limit of immunohistochemistry and is far beyond that of conventional histology. Our results emphasise the importance of PCR to complement routine histology of cutaneous leishmaniasis cases, particularly in laboratories in which no immunohistochemical assay is available.
本报告描述了一种基于实时PCR的程序，用于可靠地确定来自犬类和马类皮肤利什曼病病例的组织学皮肤切片中利什曼原虫无鞭毛体的数量与宿主组织数量的关系。新型诊断性利什曼原虫PCR的检测限为每微克组织<0.02个无鞭毛体，这与免疫组织化学的检测限相当，且远高于传统组织学的检测限。我们的结果强调了PCR在补充皮肤利什曼病病例常规组织学方面的重要性，特别是在没有免疫组织化学检测方法的实验室中。
