Lippes J, Wagh P V
Department of Gynecology and Obstetrics, School of Medicine, State University of New York, Buffalo.
Fertil Steril. 1989 Jan;51(1):89-94. doi: 10.1016/s0015-0282(16)60434-x.
In the management of infertile patients, it is well recognized that gamete intrafallopian transfer (GIFT) has a higher success rate than in vitro fertilization and embryo transfer. Oviducts produce unique proteins that may be responsible for the success of GIFT. Unique proteins from human oviductal fluid (hOF) have been purified in the authors' laboratory. One of these proteins of 54 kDa molecular weight, and containing carbohydrate, was obtained in a highly purified state. In this study, human sperm were incubated with: (1) a mixture of hOF-specific proteins, and (2) the highly purified hOF glycoprotein, designated human oviductin I (hOV-I). Using indirect immunofluorescence, the authors studied the ability of hOF proteins to bind to human sperm. The mixture of hOF proteins appeared to bind to the surface of the entire sperm. Conversely, hOV-I binding was restricted to the head region only. Studies are in progress to discern the function of the sperm-binding interaction(s) with hOF proteins.
在不育患者的治疗中，人们普遍认识到配子输卵管内移植（GIFT）的成功率高于体外受精和胚胎移植。输卵管会产生独特的蛋白质，这可能是GIFT成功的原因。作者所在实验室已从人输卵管液（hOF）中纯化出独特的蛋白质。其中一种分子量为54 kDa且含有碳水化合物的蛋白质已达到高度纯化状态。在本研究中，将人类精子与以下物质孵育：（1）hOF特异性蛋白质混合物，以及（2）高度纯化的hOF糖蛋白，命名为人输卵管蛋白I（hOV-I）。作者利用间接免疫荧光法研究了hOF蛋白质与人类精子结合的能力。hOF蛋白质混合物似乎能结合到整个精子表面。相反，hOV-I的结合仅局限于头部区域。目前正在进行研究以探究精子与hOF蛋白质结合相互作用的功能。
