Wagh P V, Lippes J
Department of Biochemical Pharmacology, School of Pharmacy, State University of New York, Buffalo.
Fertil Steril. 1993 Jan;59(1):148-56. doi: 10.1016/s0015-0282(16)55631-3.
To investigate whether human oviductin-I (hOV-I) from human oviductal fluid (hOF) and human alpha-fetoprotein (hAFP) are identical molecules.
Comparison of amino acid and carbohydrate composition of hOV-I with that of hAFP. Isolation of hAFP from oviductal fluid of a patient and evaluation of its immunochemical properties in relation to hOV-I and authentic hAFP standard.
Procedures were performed in an academic research environment.
Human oviductin-I was isolated from a pooled sample of hOF obtained from four patients in a third world country. AFP was purified from a sample of oviductal fluid obtained from a single patient during her periovulatory period.
Human oviductal fluid was collected coincident to elective tubal ligations.
The amino acid and carbohydrate composition of hOV-I was determined. AFP was purified from hOF using ion-exchange chromatography and gel filtration. The identity between hOV-I and hAFP from hOF was assessed using enzyme-linked immunosorbent assay (ELISA) and Western immunoblot analysis.
The amino acid and carbohydrate composition of hOV-I indicated similarities between hOV-I and hAFP. Both hOV-I and hAFP reacted with mouse anti-hAFP monoclonal antibody and purified polyclonal rabbit anti-hOV-I immunoglobulin G in an identical manner as observed by noncompetitive ELISA. Purified AFP from hOF of a single patient had a molecular mass of 66 kd and was found to be identical with hOV-I by Western immunoblot analysis.
Human oviductin-I and hAFP are chemically similar and immunologically identical molecules. We believe that this finding is the first documentation for the existence of AFP in hOF.
研究来自人输卵管液（hOF）的人输卵管素-I（hOV-I）与人甲胎蛋白（hAFP）是否为同一分子。
比较hOV-I与hAFP的氨基酸和碳水化合物组成。从一名患者的输卵管液中分离hAFP，并评估其与hOV-I和正宗hAFP标准品相关的免疫化学特性。
在学术研究环境中进行实验步骤。
人输卵管素-I从一个第三世界国家的4名患者的hOF混合样本中分离得到。AFP从一名患者排卵期的输卵管液样本中纯化得到。
在择期输卵管结扎术时收集人输卵管液。
测定hOV-I的氨基酸和碳水化合物组成。使用离子交换色谱法和凝胶过滤法从hOF中纯化AFP。使用酶联免疫吸附测定（ELISA）和Western免疫印迹分析评估hOF中hOV-I和hAFP之间的同一性。
hOV-I的氨基酸和碳水化合物组成表明hOV-I与hAFP之间存在相似性。hOV-I和hAFP与小鼠抗hAFP单克隆抗体以及纯化的兔抗hOV-I多克隆免疫球蛋白G的反应方式相同，这在非竞争性ELISA中可见。从一名患者的hOF中纯化的AFP分子量为66kd，通过Western免疫印迹分析发现其与hOV-I相同。
人输卵管素-I和hAFP在化学上相似且在免疫学上为同一分子。我们认为这一发现是hOF中存在AFP的首次记录。
