Zhao Chen, Su Guangyue, Wang Xude, Zhang Xiaoshu, Guo Shuang, Zhao Yuqing
Shenyang Pharmaceutical University, Shenyang, 110016, People's Republic of China.
Biotechnol Lett. 2016 Jan;38(1):43-50. doi: 10.1007/s10529-015-1964-4. Epub 2015 Oct 1.
25-Hydroxyprotopanaxadiol (25-OH-PPD) and 25-methoxylprotopanaxadiol (25-OCH3-PPD), two ginseng sapogenins, have potent antitumor activity and their effects on gastric cancer (BGC-823, SGC-7901, MKN-28) cells and a gastric mucosa (GES-1) cell line are reported.
Both compounds significantly inhibited the growth of gastric cancer cells, while having lesser inhibitory effects on GES-1 cells by MTT assay. A mechanistic study revealed that the two ginseng sapogenins could induce apoptosis in BGC-823 cells by morphological observation, DNA fragmentation, flow cytometry and western blot analysis. Besides, the apoptosis was inhibited by Ac-DEVD-CHO, a caspase 3 inhibitor, which was confirmed by cell viability analysis.
These results indicate that 25-OH-PPD and 25-OCH3-PPD have potential to be promising agents for the treatment of gastric cancer.
人参二醇型皂苷25-羟基原人参二醇（25-OH-PPD）和25-甲氧基原人参二醇（25-OCH3-PPD）具有强大的抗肿瘤活性，本文报道了它们对胃癌（BGC-823、SGC-7901、MKN-28）细胞系及胃黏膜（GES-1）细胞系的作用。
通过MTT法检测发现，这两种化合物均能显著抑制胃癌细胞的生长，而对GES-1细胞的抑制作用较小。机制研究表明，通过形态学观察、DNA片段化分析、流式细胞术及蛋白质免疫印迹分析发现，这两种人参二醇型皂苷可诱导BGC-823细胞凋亡。此外，细胞活力分析证实，凋亡可被半胱天冬酶3抑制剂Ac-DEVD-CHO抑制。
这些结果表明，25-OH-PPD和25-OCH3-PPD有潜力成为治疗胃癌的有效药物。
