人参皂苷元、25-羟基原人参二醇和25-甲氧基原人参二醇对胃癌细胞的抗肿瘤活性。
Antitumor activity of ginseng sapogenins, 25-OH-PPD and 25-OCH3-PPD, on gastric cancer cells.
作者信息
Zhao Chen, Su Guangyue, Wang Xude, Zhang Xiaoshu, Guo Shuang, Zhao Yuqing
机构信息
Shenyang Pharmaceutical University, Shenyang, 110016, People's Republic of China.
出版信息
Biotechnol Lett. 2016 Jan;38(1):43-50. doi: 10.1007/s10529-015-1964-4. Epub 2015 Oct 1.
OBJECTIVES
25-Hydroxyprotopanaxadiol (25-OH-PPD) and 25-methoxylprotopanaxadiol (25-OCH3-PPD), two ginseng sapogenins, have potent antitumor activity and their effects on gastric cancer (BGC-823, SGC-7901, MKN-28) cells and a gastric mucosa (GES-1) cell line are reported.
RESULTS
Both compounds significantly inhibited the growth of gastric cancer cells, while having lesser inhibitory effects on GES-1 cells by MTT assay. A mechanistic study revealed that the two ginseng sapogenins could induce apoptosis in BGC-823 cells by morphological observation, DNA fragmentation, flow cytometry and western blot analysis. Besides, the apoptosis was inhibited by Ac-DEVD-CHO, a caspase 3 inhibitor, which was confirmed by cell viability analysis.
CONCLUSIONS
These results indicate that 25-OH-PPD and 25-OCH3-PPD have potential to be promising agents for the treatment of gastric cancer.
目的
人参二醇型皂苷25-羟基原人参二醇（25-OH-PPD）和25-甲氧基原人参二醇（25-OCH3-PPD）具有强大的抗肿瘤活性，本文报道了它们对胃癌（BGC-823、SGC-7901、MKN-28）细胞系及胃黏膜（GES-1）细胞系的作用。
结果
通过MTT法检测发现，这两种化合物均能显著抑制胃癌细胞的生长，而对GES-1细胞的抑制作用较小。机制研究表明，通过形态学观察、DNA片段化分析、流式细胞术及蛋白质免疫印迹分析发现，这两种人参二醇型皂苷可诱导BGC-823细胞凋亡。此外，细胞活力分析证实，凋亡可被半胱天冬酶3抑制剂Ac-DEVD-CHO抑制。
结论
这些结果表明，25-OH-PPD和25-OCH3-PPD有潜力成为治疗胃癌的有效药物。
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