Song Chaoran, Shen Ting, Kim Han Gyung, Hu Weicheng, Cho Jae Youl
Department of Integrative Biotechnology, Biomedical Institute for Convergence at SKKU (BICS), Sungkyunkwan University, Suwon 16419, Korea.
Jiangsu Collaborative Innovation Center of Regional, Modern Agriculture & Environmental Protection, Jiangsu Key Laboratory for Eco-Agricultural, Biotechnology Around Hongze Lake, Huaiyin Normal University, Huaian 223300, P. R. China.
Am J Chin Med. 2023;51(1):205-221. doi: 10.1142/S0192415X2350012X. Epub 2022 Nov 21.
20(S)-protopanaxadiol (PPD), a metabolite of , has multiple pharmacological properties. However, the effects of PPD against human gastric cancer have not been elucidated. Our purpose in this study was to investigate if PPD has anticancer effects against human gastric cancer . Cell viability, migration, clone formation, and invasion were assessed to explore the effects of PPD on cancer cells. PI and annexin V staining as well as immunoblotting were employed to determine if PPD-induced apoptosis and autophagy of MKN1 and MKN45 cells. The target of PPD was identified using immunoblotting, overexpression analysis, and flow cytometric analysis. PPD exhibited significantly suppressed cell viability, migration, colony formation, and invasion. Phosphorylation of Src and its down-stream effectors were inhibited by PPD. PPD-enhanced apoptosis and autophagy in a dose- and time-dependent manner by inhibiting Src. Collectively, our results demonstrate that PPD induces apoptosis and autophagy in gastric cancer cells by inhibiting Src.
20(S)-原人参二醇（PPD）是[某种物质]的一种代谢产物，具有多种药理特性。然而，PPD对人胃癌的作用尚未阐明。本研究的目的是探讨PPD是否对人胃癌具有抗癌作用。通过评估细胞活力、迁移、克隆形成和侵袭来探究PPD对癌细胞的影响。采用PI和膜联蛋白V染色以及免疫印迹法来确定PPD是否诱导MKN1和MKN45细胞凋亡和自噬。使用免疫印迹法、过表达分析和流式细胞术分析来鉴定PPD的作用靶点。PPD显著抑制细胞活力、迁移、集落形成和侵袭。PPD抑制Src及其下游效应器的磷酸化。PPD通过抑制Src以剂量和时间依赖性方式增强凋亡和自噬。总的来说，我们的结果表明PPD通过抑制Src诱导胃癌细胞凋亡和自噬。
