Wang Guangqiang, Xia Yongjun, Song Xin, Ai Lianzhong
School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, People's Republic of China.
Curr Microbiol. 2016 Jan;72(1):102-11. doi: 10.1007/s00284-015-0915-6. Epub 2015 Oct 1.
The non-classically secreted proteins are the proteins that are located in the extracellular milieu despite the absence of known signal peptides or other targeting peptides. Numerous experimental studies have discovered many non-classically secreted proteins, but there are many inconsistencies in these reports even on a specific organism. In order to reduce the impact of artefactual contamination of protein samples and cell lysis, this review listed 45 common non-classically secreted proteins. The list of non-classically secreted proteins was complied from studies carried out by different groups in various bacterial species. The complied list can provide a reliable experimental material for further research on non-classical protein secretion. It is noticeable that some of these proteins are hyper-secreted when expressed in specific species or under some induction conditions. So we also discussed the possibilities of using the non-classical secretion pathway for the production of recombinant proteins in the review.
非经典分泌蛋白是指那些尽管没有已知的信号肽或其他靶向肽，但仍位于细胞外环境中的蛋白质。众多实验研究已经发现了许多非经典分泌蛋白，但即使是针对特定生物体的这些报告也存在许多不一致之处。为了减少蛋白质样品人为污染和细胞裂解的影响，本综述列出了45种常见的非经典分泌蛋白。非经典分泌蛋白列表是根据不同研究小组在各种细菌物种中开展的研究汇编而成的。该汇编列表可为非经典蛋白质分泌的进一步研究提供可靠的实验材料。值得注意的是，其中一些蛋白质在特定物种中表达或在某些诱导条件下会过度分泌。因此，我们在综述中还讨论了利用非经典分泌途径生产重组蛋白的可能性。
