Börner G Valentin, Cha Rita S
Center for Gene Regulation in Health and Disease, Department of Biological, Geological and Environmental Sciences, Cleveland State University, Cleveland, Ohio 44115-2214;
North West Cancer Research Institute, School of Medical Sciences, Bangor University, Bangor LL57 2UW, United Kingdom.
Cold Spring Harb Protoc. 2015 Oct 1;2015(10):914-24. doi: 10.1101/pdb.prot085050.
During meiosis, one round of genome duplication is followed by two rounds of chromosome segregation, resulting in the halving of the genetic complement and the formation of haploid reproductive cells. In most organisms, intimate juxtaposition of homologous chromosomes and homologous recombination during meiotic prophase are required for meiotic success. Here we present a general protocol for visualizing chromosomal proteins and homolog interaction on surface-spread nuclei and a widely used protocol for analyzing meiotic recombination based on an engineered hotspot referred to as HIS4::LEU2.
在减数分裂过程中，一轮基因组复制之后是两轮染色体分离，导致遗传物质减半并形成单倍体生殖细胞。在大多数生物体中，减数分裂前期同源染色体的紧密并列和同源重组是减数分裂成功所必需的。在这里，我们展示了一种用于在表面铺展的细胞核上可视化染色体蛋白和同源物相互作用的通用方案，以及一种基于称为HIS4::LEU2的工程热点来分析减数分裂重组的广泛使用的方案。
