Heikkinen Anna Maria, Nwhator Solomon O, Rathnayake Nilminie, Mäntylä Päivi, Vatanen Päivi, Sorsa Timo
Department of Oral and Maxillofacial Diseases, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.
Department of Preventive and Community Dentistry, Faculty of Dentistry, College of Health Sciences, Obafemi Awolowo University, Ife, Nigeria.
J Periodontol. 2016 Jan;87(1):36-40. doi: 10.1902/jop.2015.150377. Epub 2015 Oct 2.
Matrix metalloproteinase (MMP)-8 is a major destructive collagenase involved in periodontitis and can be regarded as a periodontitis biomarker. A neutrophil collagenase 2 (active MMP-8 [aMMP-8]) oral fluid immunoassay has recently been demonstrated to be a periodontitis risk indicator among adults. The aim of this study is to investigate whether a point-of-care mouthrinse test based on an aMMP-8 immunoassay could identify patients with oral inflammatory burden (periodontitis and caries) among adolescents with early pathologic findings.
This cross-sectional study was carried out at the Kotka Health Center, Finland. First, the aMMP-8 chairside mouthrinse test was performed on enrolled individuals (adolescents aged 15 to 17 years, n = 47), and the results were read based on a color change within 5 minutes. Then, full-mouth clinical parameters of oral health were assessed, including periodontal, oral mucosal, and caries status.
The sensitivity and specificity of the test for bleeding on probing were 71.8% and 77.5%, respectively (P = 0.05); for ≥1 site with probing depth (PD) ≥4 mm, 48.3% and 100% (P <0.001); for ≥2 sites with PD ≥4 mm, 63.6% and 100% (P <0.001); and for >2 sites with PD ≥4 mm, 76.5% and 96.7% (P <0.01). Regarding periodontitis (≥1 site with PD ≥4 mm), hardly any false-positive results were identified. The sensitivity of the immunoassay for ≥1 caries lesions was 76.5%, and the specificity was 96.7% (P <0.01).
In 5 minutes, the aMMP-8 chairside test showed promising results, recognizing oral inflammatory burden in adolescents with early initial signs of periodontitis. Caries lesions could also be detected, but less efficiently.
基质金属蛋白酶（MMP）-8是参与牙周炎的一种主要破坏性胶原酶，可被视为牙周炎生物标志物。最近已证明，中性粒细胞胶原酶2（活性MMP-8 [aMMP-8]）口腔液免疫测定法是成人牙周炎风险指标。本研究的目的是调查基于aMMP-8免疫测定的即时护理漱口水测试能否识别有早期病理表现的青少年中患有口腔炎症负担（牙周炎和龋齿）的患者。
这项横断面研究在芬兰科特卡健康中心进行。首先，对纳入个体（15至17岁青少年，n = 47）进行aMMP-8椅旁漱口水测试，并根据5分钟内的颜色变化读取结果。然后，评估口腔健康的全口临床参数，包括牙周、口腔黏膜和龋齿状况。
该测试对探诊出血的敏感性和特异性分别为71.8%和77.5%（P = 0.05）；对于≥1个探诊深度（PD）≥4 mm的部位，分别为48.3%和100%（P <0.001）；对于≥2个PD≥4 mm的部位，分别为63.6%和100%（P <0.001）；对于>2个PD≥4 mm的部位，分别为76.5%和96.7%（P <0.01）。关于牙周炎（≥1个部位PD≥4 mm），几乎未发现假阳性结果。该免疫测定法对≥1个龋损的敏感性为76.5%，特异性为96.7%（P <0.01）。
在5分钟内，aMMP-8椅旁测试显示出有前景的结果，可识别有牙周炎早期初始迹象的青少年的口腔炎症负担。龋损也能被检测到，但效率较低。
