Goodwin R G, Lupton S, Schmierer A, Hjerrild K J, Jerzy R, Clevenger W, Gillis S, Cosman D, Namen A E
Immunex Corporation, Seattle, WA 98101.
Proc Natl Acad Sci U S A. 1989 Jan;86(1):302-6. doi: 10.1073/pnas.86.1.302.
A cDNA encoding biologically active human interleukin 7 was isolated by hybridization with the homologous murine clone. Nucleotide sequence analysis indicated that this cDNA was capable of encoding a protein of 177 amino acids with a signal sequence of 25 amino acids and a calculated mass of 17.4 kDa for the mature protein. Recombinant human interleukin 7 stimulated the proliferation of murine pre-B cells and was active on cells harvested from human bone marrow that are enriched for B-lineage progenitor cells. Analysis of RNA by blot hybridization demonstrated the presence of two size classes of interleukin 7 mRNA in human splenic and thymic tissue.
通过与同源小鼠克隆杂交,分离出了编码具有生物活性的人白细胞介素7的cDNA。核苷酸序列分析表明,该cDNA能够编码一种由177个氨基酸组成的蛋白质,其信号序列为25个氨基酸,成熟蛋白的计算分子量为17.4 kDa。重组人白细胞介素7刺激小鼠前B细胞的增殖,并且对从富含B系祖细胞的人骨髓中收获的细胞具有活性。通过印迹杂交分析RNA表明,在人脾脏和胸腺组织中存在两种大小类别的白细胞介素7 mRNA。